| Application of porcine lipase secreted by pichia pastoris to improve fat digestion and growth performance of postweaning piglets. | |
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MedLine Citation:
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PMID: 20166658 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the study was to use Pichia pastoris to express a recombinant porcine lipase gene (pLip). The expression-secretion cassette was constructed using the P. pastoris GAPDH (glyceraldehyde-3-phosphate-dehydrogenase) promoter and an 89-residue prepro-alpha-factor secretion signal fused to the AOX1 terminator (the pGAPZalphaA vector). A total of 1,408 bp of pancreatic lipase cDNA was produced, which was located from the position of 4-nt upstream of ATG to 1408-nt inside the intact coding region of the pLip sequence. In an animal trial, three concentrations of recombinant lipase activity (0, 5,000 and 10,000 U/kg) were blended with the basal diet and fed to weaned piglets for six weeks. During the experimental period, the growth performance (bodyweight, feed intake, and feed efficiency) of the test groups was superior to that of the control group (p < 0.05). Furthermore, the group fed the diet blended with 10,000 U/kg of recombinant lipase showed significant (p < 0.05) increases in blood triglyceride (TG) concentration on the seventh day postweaning. These results suggested that the porcine lipase protein yielded by transformed yeast cells may improve fat digestibility and enhance the growth performance in postweaning piglets. |
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Authors:
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Fang-Chueh Liu; Hsiao-Ling Chen; Willie Lin; Yu-Tang Tung; Cheng-Wei Lai; A-Li Hsu; Chuan-Mu Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-17 Completed Date: 2010-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 3322-9 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis Animals Dietary Supplements / analysis* Digestion* Enzyme Stability Fats / metabolism* Female Fungal Proteins / chemistry, genetics, metabolism* Lipase / chemistry, genetics, metabolism* Male Pichia / enzymology* Protein Engineering* Protein Transport Swine / growth & development, physiology* Weaning |
| Chemical | |
Reg. No./Substance:
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0/Fats; 0/Fungal Proteins; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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