Document Detail

Application of Plackett-Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16.
MedLine Citation:
PMID:  17846761     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to use statistically based experimental designs for the optimization of xylanase production from Alternaria mali ND-16. Ten components in the medium were screened for nutritional requirements. Three nutritional components, including NH(4)Cl, urea, and MgSO(4), were identified to significantly affect the xylanase production by using the Plackett-Burman experimental design. These three major components were subsequently optimized using the Doehlert experimental design. By using response surface methodology and canonical analysis, the optimal concentrations for xylanase production were: NH(4)Cl 11.34 g L(-1), urea 1.26 g L(-1), and MgSO(4) 0.98 g L(-1). Under these optimal conditions, the xylanase activity from A. mali ND-16 reached 30.35 U mL(-1). Verification of the optimization showed that xylanase production of 31.26 U mL(-1) was achieved.
Yin Li; Zhiqiang Liu; Fengjie Cui; Zhisheng Liu; Hui Zhao
Publication Detail:
Type:  Journal Article     Date:  2007-09-11
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  77     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-29     Completed Date:  2008-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  285-91     Citation Subset:  IM    
Department of Plant Science, North Dakota State University, Fargo, ND 58105, USA.
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MeSH Terms
Alternaria / classification,  enzymology*,  genetics,  growth & development*
Ammonium Chloride / metabolism
Culture Media / chemistry*
Food Technology / methods
Magnesium Sulfate / metabolism
Molecular Sequence Data
Research Design
Sequence Analysis, DNA
Soil Microbiology*
Urea / metabolism
Xylans / metabolism
Xylosidases / biosynthesis*,  metabolism
Reg. No./Substance:
0/Culture Media; 0/Xylans; 12125-02-9/Ammonium Chloride; 57-13-6/Urea; 7487-88-9/Magnesium Sulfate; EC 3.2.1.-/Xylosidases; EC,4-beta-D-xylosidase

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