| Application of FLIM-FIDSAM for the in vivo analysis of hormone competence of different cell types. | |
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MedLine Citation:
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PMID: 20811880 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Background fluorescence derived from subcellular compartments is a major drawback in high-resolution live imaging, especially of plant cells. A novel technique for contrast enhancement of fluorescence images of living cells expressing fluorescent fusion proteins termed fluorescence intensity decay shape analysis microscopy (FIDSAM) has been recently published and is applied here to plant cells expressing wild-type levels of a low-abundant membrane protein (BRI1-EGFP), demonstrating the applicability of FIDSAM to samples exhibiting about 80% autofluorescence. Furthermore, the combination of FIDSAM and fluorescence lifetime imaging microscopy enables the simultaneous determination and quantification of different ligand-specific responses in living cells with high spatial and temporal resolution even in samples with high autofluorescence background. Correlation of different responses can be used to determine the hormone ligand competence of different cell types as demonstrated here in BRI1-EGFP-expressing root and hypocotyl cells. |
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Authors:
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Kirstin Elgass; Katharina Caesar; Dierk Wanke; Klaus Harter; Alfred J Meixner; Frank Schleifenbaum |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-02 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 398 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1919-25 Citation Subset: IM |
Affiliation:
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Institute of Physical and Theoretical Chemistry, University of Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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