Document Detail


Application of drag-reducing polymer solutions as test fluids for in vitro evaluation of potential blood damage in blood pumps.
MedLine Citation:
PMID:  20019596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vitro evaluation of the potential of a circulatory-assist device to damage blood cells has generally been performed using blood from various species. Problems with this approach include the variability of blood sensitivity to mechanical stress in different species, preparation of blood including the adjustment of hematocrit to a standard value, changes in the mechanical properties of blood that occur during storage, and necessity to pool blood samples to obtain an adequate amount of blood for in vitro circulating systems. We investigated whether the mechanical degradation of a drag-reducing polymer (DRP) solution resulting in the loss of drag-reducing ability can indicate the degree of shear-induced blood damage within blood pumps. DRP solution (polyethylene oxide, 4,500 kDa, 1,000 ppm) or porcine blood were driven through a turbulent flow system by a centrifugal pump, either the Bio-Pump BPX-80 (Medtronic, Inc.) or CentriMag (Levitronix LLC) at a constant pressure gradient of 300 mm Hg for 120 minutes. DRP mechanical degradation was evaluated by reduction of flow rate and solution viscosity. A proposed index of DRP mechanical degradation (PDI) is similar to the normalized index of hemolysis (NIH) typically used to quantify the results of in vitro testing of blood pumps. Results indicate that the mechanical degradation of DRP solutions may provide a sensitive standard method for the evaluation of potential blood trauma produced by blood pumps without the use of blood.
Authors:
Amanda R Daly; Hideo Sobajima; Salim E Olia; Setsuo Takatani; Marina V Kameneva
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  ASAIO journal (American Society for Artificial Internal Organs : 1992)     Volume:  56     ISSN:  1538-943X     ISO Abbreviation:  ASAIO J.     Publication Date:    2010 Jan-Feb
Date Detail:
Created Date:  2010-01-14     Completed Date:  2010-03-09     Revised Date:  2011-07-20    
Medline Journal Info:
Nlm Unique ID:  9204109     Medline TA:  ASAIO J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6-11     Citation Subset:  IM    
Affiliation:
McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Heart-Assist Devices / adverse effects*
Polyethylene Glycols*
Rheology / methods*
Stress, Mechanical*
Swine
Grant Support
ID/Acronym/Agency:
R01 HL089456-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Polyethylene Glycols
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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