Document Detail


Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis.
MedLine Citation:
PMID:  12952873     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yersinia pestis, the causative agent of plague, diverged from Yersinia pseudotuberculosis, an enteric pathogen, an estimated 1500-20,000 years ago. Genetic characterization of these closely related organisms represents a useful model to study the rapid emergence of bacterial pathogens that threaten mankind. To this end, we undertook genome-wide DNA microarray analysis of 22 strains of Y. pestis and 10 strains of Y. pseudotuberculosis of diverse origin. Eleven Y. pestis DNA loci were deemed absent or highly divergent in all strains of Y. pseudotuberculosis. Four were regions of phage origin, whereas the other seven included genes encoding a vitamin B12 receptor and the insect toxin sepC. Sixteen differences were identified between Y. pestis strains, with biovar Antiqua and Mediaevalis strains showing most divergence from the arrayed CO92 Orientalis strain. Fifty-eight Y. pestis regions were specific to a limited number of Y. pseudotuberculosis strains, including the high pathogenicity island, three putative autotransporters, and several possible insecticidal toxins and hemolysins. The O-antigen gene cluster and one of two possible flagellar operons had high levels of divergence between Y. pseudotuberculosis strains. This study reports chromosomal differences between species, biovars, serotypes, and strains of Y. pestis and Y. pseudotuberculosis that may relate to the evolution of these species in their respective niches.
Authors:
Stewart J Hinchliffe; Karen E Isherwood; Richard A Stabler; Michael B Prentice; Alexander Rakin; Richard A Nichols; Petra C F Oyston; Jason Hinds; Richard W Titball; Brendan W Wren
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Genome research     Volume:  13     ISSN:  1088-9051     ISO Abbreviation:  Genome Res.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-03     Completed Date:  2003-10-20     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9518021     Medline TA:  Genome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2018-29     Citation Subset:  IM    
Affiliation:
The London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / genetics
Animals
Antigens, Bacterial / genetics
Bacterial Adhesion / genetics
Bacterial Toxins / genetics
Chromosomes, Bacterial / genetics
Evolution, Molecular*
Gene Expression Regulation, Bacterial / genetics
Genetic Markers
Genetic Variation / genetics
Genome, Bacterial*
Genomics / methods*
Humans
Insects / microbiology
Oligonucleotide Array Sequence Analysis / methods*
Species Specificity
Virulence / genetics
Yersinia pestis / classification,  genetics*,  pathogenicity
Yersinia pseudotuberculosis / classification,  genetics*,  pathogenicity
Grant Support
ID/Acronym/Agency:
062511//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Toxins; 0/Genetic Markers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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