Document Detail


Application of DNA chip scanning technology for the automatic detection of Chlamydia trachomatis and Chlamydia pneumoniae inclusions.
MedLine Citation:
PMID:  24189259     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chlamydiae are obligate intracellular bacteria that propagate in the inclusion, a specific niche inside the host cell. The standard method for counting chlamydiae is the immunofluorescent staining and manual counting of chlamydial inclusions. High or medium throughput estimation of the reduction in chlamydia inclusions should be the basis of testing antichlamydial compounds and other drugs that positively or negatively influence chlamydial growth, yet low-throughput manual counting is the common approach. To overcome the time-consuming and subjective manual counting we developed an automatic inclusion counting system based on a commercially available DNA chip scanner. Fluorescently labeled inclusions are detected by the scanner, and the image is processed by ChlamyCount, a custom plugin of the ImageJ software environment. ChlamyCount was able to measure the inclusion counts over a one log dynamic range with high correlation to the theoretical counts. ChlamyCount was capable of accurately determining the minimum inhibitory concentration of the novel antimicrobial compound PCC00213 and the already known antichlamydial antibiotics moxifloxacin and tetracycline. ChlamyCount was also able to measure the chlamydial growth altering effect of drugs that influence host-bacterium interaction such as interferon-gamma, DEAE-dextran and cycloheximide. ChlamyCount is an easily adaptable system for testing antichlamydial antimicrobials and other compounds that influence Chlamydia-host interactions.
Authors:
Anita Bogdanov; Valeria Endrész; Szabolcs Urbán; Ildikó Lantos; Judit Deák; Katalin Burián; Kamil Onder; Ferhan Ayaydin; Péter Balázs; Dezso P Virok
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-11-4
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  -     ISSN:  1098-6596     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary Address: H-6725 Szeged Semmelweis str. 6.
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