Document Detail


[Application of DNA extraction kit, 'GM quicker' for detection of genetically modified soybeans].
MedLine Citation:
PMID:  22450668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several DNA extraction methods have been officially introduced to detect genetically modified soybeans, but the choice of DNA extraction kits depend on the nature of the samples, such as grains or processed foods. To overcome this disadvantage, we examined whether the GM quicker kit is available for both grains and processed foods. We compared GM quicker with four approved DNA extraction kits in respect of DNA purity, copy numbers of lectin gene, and working time. We found that the DNA quality of GM quicker was superior to that of the other kits for grains, and the procedure was faster. However, in the case of processed foods, GM quicker was not superior to the other kits. We therefore investigated an unapproved GM quicker 3 kit, which is available for DNA extraction from processed foods, such as tofu and boiled soybeans. The GM quicker 3 kit provided good DNA quality from both grains and processed foods, so we made a minor modification of the GM quicker-based protocol that was suitable for processed foods, using GM quicker and its reagents. The modified method enhanced the performance of GM quicker with processed foods. We believe that GM quicker with the modified protocol is an excellent tool to obtain high-quality DNA from grains and processed foods for detection of genetically modified soybeans.
Authors:
Noriko Sato; Yoshitsugu Sugiura; Toshitsugu Tanaka
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Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan     Volume:  53     ISSN:  1882-1006     ISO Abbreviation:  Shokuhin Eiseigaku Zasshi     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-08-28     Revised Date:  2013-03-11    
Medline Journal Info:
Nlm Unique ID:  0142214     Medline TA:  Shokuhin Eiseigaku Zasshi     Country:  Japan    
Other Details:
Languages:  jpn     Pagination:  39-44     Citation Subset:  IM    
Affiliation:
Department of Food Chemistry, Kobe Institute of Health,Kobe, Japan. noriko_sato@office.city.kobe.lg.jp
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MeSH Terms
Descriptor/Qualifier:
DNA, Plant / analysis*
Food, Genetically Modified*
Genetic Techniques / instrumentation
Plants, Genetically Modified / genetics
Polymerase Chain Reaction
Soybeans / genetics*
Chemical
Reg. No./Substance:
0/DNA, Plant
Comments/Corrections
Erratum In:
Shokuhin Eiseigaku Zasshi. 2012;53(2):128

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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