Document Detail

Application of the CYTOMIC 12 flow cytometric compact analyzer for automatic kinetic measurements.
MedLine Citation:
PMID:  6822145     Owner:  NLM     Status:  MEDLINE    
Flow cytometry, usually applied to cells which have time independent features, can also be used for kinetic experiments where the change of cell populations with time is investigated. Dedicated time sequencing programs written in Assembler and incorporated in the CYTOMIC 12 analyzer (4) are described. A sequence of 64 one parameter histograms can be automatically acquired and immediately displayed as a pseudo-two-parameter histogram. The acquisition time for each of the subsequent histograms can be selected between 1 and 32 seconds. Kinetics lasting up to 34 minutes are resolved into 64 time intervals. Two parameter kinetics can be resolved into 12 32 X 32 channel, two parameter histograms which are displayed and evaluated immediately on the analyzer screen in groups of 4 without using complicated list mode procedures. The standard CYTOMIC 12 software can be applied for processing and printing of the sequence distribution curves.
V Kachel; H Schneider; J Bauer; J Malin-Berdel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry     Volume:  3     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-03-24     Completed Date:  1983-03-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  244-50     Citation Subset:  IM    
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MeSH Terms
Bone Marrow Cells
Data Display
Flow Cytometry / instrumentation,  methods*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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