Document Detail


Application of the CYTOMIC 12 flow cytometric compact analyzer for automatic kinetic measurements.
MedLine Citation:
PMID:  6822145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flow cytometry, usually applied to cells which have time independent features, can also be used for kinetic experiments where the change of cell populations with time is investigated. Dedicated time sequencing programs written in Assembler and incorporated in the CYTOMIC 12 analyzer (4) are described. A sequence of 64 one parameter histograms can be automatically acquired and immediately displayed as a pseudo-two-parameter histogram. The acquisition time for each of the subsequent histograms can be selected between 1 and 32 seconds. Kinetics lasting up to 34 minutes are resolved into 64 time intervals. Two parameter kinetics can be resolved into 12 32 X 32 channel, two parameter histograms which are displayed and evaluated immediately on the analyzer screen in groups of 4 without using complicated list mode procedures. The standard CYTOMIC 12 software can be applied for processing and printing of the sequence distribution curves.
Authors:
V Kachel; H Schneider; J Bauer; J Malin-Berdel
Related Documents :
3880325 - Seq-ed: an interactive computer program for editing, analysis and storage of long dna s...
9461455 - Automating the identification of dna variations using quality-based fluorescence re-seq...
16767765 - General algorithm for automated off-center mri.
15699025 - A comparative analysis of relative occurrence of transcription factor binding sites in ...
2974535 - An in vitro interaction between the human u3 snrnp and 28s rrna sequences near the alph...
21153745 - Five phylogenetic groups identified in the coat protein gene of grapevine leafroll-asso...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry     Volume:  3     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-03-24     Completed Date:  1983-03-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  244-50     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells
Data Display
Erythrocytes
Flow Cytometry / instrumentation,  methods*
Hemolysis
Humans
Kinetics
Rats
Sheep
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The effect of intravenous clonidine hydrochloride on the isolated forearm venous segment in heart fa...
Next Document:  Determination of linear fluorescence intensities from flow cytometric data accumulated with logarith...