| Application of the CYTOMIC 12 flow cytometric compact analyzer for automatic kinetic measurements. | |
| | |
MedLine Citation:
|
PMID: 6822145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Flow cytometry, usually applied to cells which have time independent features, can also be used for kinetic experiments where the change of cell populations with time is investigated. Dedicated time sequencing programs written in Assembler and incorporated in the CYTOMIC 12 analyzer (4) are described. A sequence of 64 one parameter histograms can be automatically acquired and immediately displayed as a pseudo-two-parameter histogram. The acquisition time for each of the subsequent histograms can be selected between 1 and 32 seconds. Kinetics lasting up to 34 minutes are resolved into 64 time intervals. Two parameter kinetics can be resolved into 12 32 X 32 channel, two parameter histograms which are displayed and evaluated immediately on the analyzer screen in groups of 4 without using complicated list mode procedures. The standard CYTOMIC 12 software can be applied for processing and printing of the sequence distribution curves. |
| | |
Authors:
|
V Kachel; H Schneider; J Bauer; J Malin-Berdel |
Related Documents
:
|
3880325 - Seq-ed: an interactive computer program for editing, analysis and storage of long dna s... 9461455 - Automating the identification of dna variations using quality-based fluorescence re-seq... 16767765 - General algorithm for automated off-center mri. 15699025 - A comparative analysis of relative occurrence of transcription factor binding sites in ... 2974535 - An in vitro interaction between the human u3 snrnp and 28s rrna sequences near the alph... 21153745 - Five phylogenetic groups identified in the coat protein gene of grapevine leafroll-asso... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Cytometry Volume: 3 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1983 Jan |
Date Detail:
|
Created Date: 1983-03-24 Completed Date: 1983-03-24 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 244-50 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bone Marrow Cells Data Display Erythrocytes Flow Cytometry / instrumentation, methods* Hemolysis Humans Kinetics Rats Sheep Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of intravenous clonidine hydrochloride on the isolated forearm venous segment in heart fa...
Next Document: Determination of linear fluorescence intensities from flow cytometric data accumulated with logarith...