Document Detail

Application of a [13CO2] breath test to study short-term amino acid catabolism during the postprandial phase of a meal.
MedLine Citation:
PMID:  17381966     Owner:  NLM     Status:  MEDLINE    
A [13CO2] breath test was applied as a non-invasive method to study the catabolism of ingested amino acids shortly after a meal. This test requires the ingestion of a [1-13C]-labelled amino acid and the analysis of expired air for [13C] enrichment and CO2. The recovery of label as [13CO2] reflects the catabolism of the [1-13C]-labelled substrate. Such a non-steady state approach provides information that is complementary to the information obtained by steady-state methods using a primed continuous infusion of tracer amino acids during the fed state. In a model study with twenty adult male rats, two groups of animals were fed twice a day with one of two semi-synthetic iso-energetic diets. One diet contained egg white protein (EW) as the sole amino acid source. The second diet contained a mixture of free amino acids with a pattern similar to that of the EW diet. On day 5 of the dietary treatment, L-[1-13C]leucine, either bound in EW protein or in free form, was ingested as part of the morning meal. The expired air was sampled at 30 min intervals for 5 h. The rate of recovery ranged from 0% to 6% of the dose/h. Up to 120 min after the onset of the meal, the recovery values for the free amino acid diet were higher than those for the EW diet. Differences in recovery reflect differences in postprandial utilisation. The differences in label recovery were mainly determined by the [13C] enrichment of the expired air. As a consequence, CO2 measurements are not mandatory when CO2 production is comparable.
Jacek Bujko; Victor V A M Schreurs; Jelmer A Nolles; Amely M Verreijen; Rudie E Koopmanschap; Martin W A Verstegen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of nutrition     Volume:  97     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-09-05     Completed Date:  2007-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  891-7     Citation Subset:  IM    
Faculty of Human Nutrition and Consumer Sciences, Warsaw Agricultural University, Poland.
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MeSH Terms
Amino Acids / administration & dosage,  analysis,  metabolism*
Body Weight / physiology
Breath Tests / methods*
Carbon Dioxide / analysis
Carbon Isotopes
Eating / physiology
Egg Proteins, Dietary / administration & dosage,  metabolism
Leucine / administration & dosage,  analysis,  metabolism
Models, Animal
Postprandial Period
Rats, Wistar
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Egg Proteins, Dietary; 124-38-9/Carbon Dioxide; 61-90-5/Leucine

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