Document Detail

Applicability of the energy dispersive x-ray microanalysis for quantification of irregular calcium deposits on fruit and leaf cuticles.
MedLine Citation:
PMID:  19094022     Owner:  NLM     Status:  MEDLINE    
In our studies, we evaluated the relation between CaCl(2) concentration and the scanning electron microscope-energy dispersive x-ray microanalysis parameters, net intensity, peak/background and standardized percentage of atoms and percentage of weight after application of single microdroplets on enzymatically isolated tomato fruit cuticles and apple seedling leaves. After analysis, the Ca/Cl ratio was calculated and the area ultimately covered with Ca and Cl within the droplet spread area determined. According to our findings, all evaluated parameters were influenced by both droplet volume and calcium chloride concentration, whereas Pearson's analysis revealed a strong correlation between net intensity and area ultimately covered by Ca or Cl, respectively. Simple linear regressions for net intensity, peak/background and Ca/Cl ratio showed variable determination coefficients (R(2)) ranging between 0.49 and 0.79. Multiple regression equations comprising net intensity, Ca/Cl ratio and deposit area were established to estimate the amount of calcium present on the cuticles. Equation slopes depended on droplet volumes, with determination coefficients of 0.89 and 0.81 for 0.5 and 1.0-microL droplets, respectively. The importance of the physicochemical properties of the spray solution was exploited in another study where a hydrophobic or a hydrophilic adjuvant was added to a 10 g L(-1) CaCl(2) solution, which was applied to apple seedling leaves. The addition of adjuvants increased values of net intensity and peak/background, which correlated significantly with the area covered by calcium. The importance of the methodology for studies on the interaction between leaf applied fertilizers (also extended to agrochemicals) and the characteristics of target surfaces is discussed.
M Hunsche; G Noga
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  232     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-19     Completed Date:  2009-01-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  453-62     Citation Subset:  IM    
Institute of Crop Science and Resource Conservation - Horticultural Science, University of Bonn,Auf dem Huegel 6, D-53121 Bonn, Germany.
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MeSH Terms
Absorptiometry, Photon / methods*
Calcium Chloride / analysis*
Fruit / chemistry*
Lycopersicon esculentum / chemistry*
Malus / chemistry*
Plant Leaves / chemistry*
Reg. No./Substance:
10043-52-4/Calcium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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