Document Detail

Apple chlorotic leaf spot virus 50 kDa movement protein acts as a suppressor of systemic silencing without interfering with local silencing in Nicotiana benthamiana.
MedLine Citation:
PMID:  17170464     Owner:  NLM     Status:  MEDLINE    
Apple chlorotic leaf spot virus (ACLSV) is the type species of the genus Trichovirus and its single-stranded, plus-sense RNA genome encodes a 216 kDa protein (P216) involved in replication, a 50 kDa movement protein (P50) and a 21 kDa coat protein (CP). In this study, it was investigated whether these proteins might have RNA silencing-suppressor activities by Agrobacterium-mediated transient assay in the green fluorescent protein-expressing Nicotiana benthamiana line 16c. The results indicated that none of these proteins could suppress local silencing in infiltrated leaves. However, systemic silencing in upper leaves induced by both single- and double-stranded RNA could be suppressed by P50, but not by a frame-shift mutant of P50, P216 or CP. Moreover, when P50 was expressed separately from where silencing signals were generated in a leaf, systemic silencing in upper leaves was inhibited. Collectively, our data indicate that P50 acts as a suppressor of systemic silencing without interfering with local silencing, probably by inhibiting the movement of silencing signals.
Hajime Yaegashi; Tsubasa Takahashi; Masamichi Isogai; Takashi Kobori; Satoshi Ohki; Nobu Yoshikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  88     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-15     Completed Date:  2007-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  316-24     Citation Subset:  IM    
The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan.
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MeSH Terms
Flexiviridae / chemistry*
Luminescent Proteins / analysis
Plant Viral Movement Proteins / physiology*
Silencer Elements, Transcriptional / physiology*
Structure-Activity Relationship
Tobacco / virology
Viral Proteins / physiology
Reg. No./Substance:
0/Luminescent Proteins; 0/Plant Viral Movement Proteins; 0/Viral Proteins

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