Document Detail

Appearance of an erythrocyte population with decreased deformability and hemoglobin content following sepsis.
MedLine Citation:
PMID:  12742829     Owner:  NLM     Status:  MEDLINE    
With the use of the cecal ligation and puncture model in mice, this study tested whether sepsis-induced decreased erythrocyte deformability is restricted to a subpopulation of cells. Erythrocyte subpopulations were isolated by centrifugal elutriation. Lineweaver-Burk conversion of deformability-response curves to shear stress was used to determine the shear stress at half-maximal cell elongation (K(EI)) and maximal cell elongation (EI(max)). Sepsis decreased erythrocyte deformability in whole blood. K(EI) values were elevated (2.7 vs. 2.1 Pa) and EI(max) values decreased (0.56 vs. 0.50) in sepsis compared with sham mice. K(EI) values for cells eluted at 7 ml/min (smallest and oldest cells) were similar; however, K(EI) values for cells eluted at 8 ml/min were greater in septic than sham animals (2.50 vs. 2.10). Younger and larger subpopulations of erythrocytes (eluted at 9, 10, and 11 ml/min) also showed a tendency of decreased deformability in sepsis. Mean corpuscular hemoglobin content was decreased in cells eluted at 7 and 8 ml/min in sepsis (4.5 and 10.2 pg) compared to sham (7.4 and 11.4 pg) mice. This study indicates that an erythrocyte subpopulation that represents 20% of circulating cells shows the most pronounced decrease in cell deformability during sepsis. Increased rigidity together with decreased corpuscular hemoglobin content in these cells may contribute to microcirculatory dysfunction and immune modulation during sepsis.
Michael R Condon; Jiyoun E Kim; Edwin A Deitch; George W Machiedo; Zoltán Spolarics
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  284     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-13     Completed Date:  2003-06-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H2177-84     Citation Subset:  IM    
Department of Surgery, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07103, USA.
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MeSH Terms
Erythrocyte Deformability / physiology*
Erythrocyte Indices
Erythrocytes / metabolism*,  physiology*
Hemoglobins / metabolism*
Image Cytometry
Indicators and Reagents
Leukocytes / metabolism
Mice, Inbred C57BL
Sepsis / blood*
Grant Support
Reg. No./Substance:
0/Hemoglobins; 0/Indicators and Reagents
Erratum In:
Am J Physiol Heart Circ Physiol. 2004 Jan;286(1):H477

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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