Document Detail


Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars.
MedLine Citation:
PMID:  10502066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendritic cells and macrophages were examined in dental pulp during the postnatal development of mouse mandibular first molars, by immuno- and enzyme histochemistry. F4/80 antibody against dendritic cells and macrophages demonstrated labeled cells predominantly in and around the odontoblastic layer during tooth development from postnatal day 0 (PN0) to PN5. Labeling with Mac-1, Mac-2, and MOMA-2 antibodies against macrophages showed varied distribution patterns. Mac-1-positive cells were not detected in the dental pulp. Mac-2-positive cells appeared in the dental pulp at PN0, but not in or around the odontoblastic layer, and disappeared by PN3. A few MOMA-2-positive cells were detected in the dental pulp during the period examined. The F4/80-positive cells in and around the odontoblastic layer did not exhibit acid phosphatase or non-specific esterase activities. In addition, the F4/80-positive cells showed continued expression of Fcgamma receptor, but not class II major histocompatibility complex (MHC). Other antibodies against dendritic cells (NLDC-145, MIDC-8, and 33D1) did not label the F4/80-positive cells. We concluded that the F4/80-positive and class II MHC-negative cells in and around the odontoblastic layer may be immature dendritic cells in the early stages before eruption, weaning, and crucial exposure to antigenic stimuli. They may not only act primarily as immunosurveillance cells, but also play a role in a regulatory function and differentiation of odontoblasts.
Authors:
E Tsuruga; Y Sakakura; T Yajima; N Shide
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  112     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-11-10     Completed Date:  1999-11-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  193-204     Citation Subset:  IM    
Affiliation:
Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan,
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis
Animals
Animals, Newborn
Antibodies, Monoclonal / immunology
Antigens, Differentiation / analysis
Carboxylesterase
Carboxylic Ester Hydrolases / analysis
Cell Count
Dendritic Cells / cytology*,  enzymology,  immunology
Dental Pulp / cytology*,  enzymology,  immunology
Galectin 3
Immunoenzyme Techniques
Immunophenotyping
Macrophage-1 Antigen / analysis
Macrophages / cytology*,  enzymology,  immunology
Mandible / embryology*,  enzymology
Mice
Mice, Inbred Strains
Molar / embryology*,  enzymology
Morphogenesis
Odontoblasts / cytology,  enzymology,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation; 0/Galectin 3; 0/Macrophage-1 Antigen; 0/monocyte-macrophage differentiation antigen; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.1/Carboxylesterase; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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