Document Detail

Appearance of denuded hepatic stellate cells and their subsequent myofibroblast-like transformation during the early stage of biliary fibrosis in the rat.
MedLine Citation:
PMID:  11810479     Owner:  NLM     Status:  MEDLINE    
To investigate the early in vivo response of hepatic stellate cells in biliary fibrosis, we examined rat livers during the first 7 days after bile duct ligation using light microscopy, immunohistochemistry, electron microscopy, and immunoelectron microscopy. At day 1 after bile duct ligation, alpha-smooth muscle actin-positive fibroblasts appeared and then increased in number around the proliferating bile ductules. With time, the destruction of the external limiting plate became accentuated because of the invasion of the proliferating bile ductules and periductural fibrosis. At day 7, stromal cells containing fat droplets appeared in the fibrous tissue adjacent to the periportal parenchyma; these are termed denuded hepatic stellate cells. In the fibrous tissue disconnected from the liver parenchyma, the denuded hepatic stellate cells were replaced by myofibroblast-like cells. Meanwhile, the expression of transforming growth factor-beta1 on biliary epithelial cells increased. These results indicate the dual origin of myofibroblasts in experimental biliary fibrosis, the periductural and periductal fibroblasts in the initial stage, and the denuded hepatic stellate cells in the subsequent stage. These two types of stromal cells may undergo myofibroblastic transformation by the transforming growth factor-beta1 secreted by the proliferating biliary epithelial cells.
L H Tao; H Enzan; Y Hayashi; E Miyazaki; T Saibara; M Hiroi; M Toi; N Kuroda; K Naruse; Y L Jin; L M Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan     Volume:  33     ISSN:  0918-4287     ISO Abbreviation:  Med Electron Microsc     Publication Date:  2000  
Date Detail:
Created Date:  2002-01-29     Completed Date:  2002-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430789     Medline TA:  Med Electron Microsc     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  217-30     Citation Subset:  IM    
First Department of Pathology, Kochi Medical School, Kohasu, Okocho, Nankoku, Kochi 783-8505, Japan.
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MeSH Terms
Actins / analysis
Bile Ducts / pathology*
Bilirubin / blood
Liver / pathology*,  ultrastructure
Microscopy, Electron
Rats, Wistar
Transforming Growth Factor beta / analysis
Reg. No./Substance:
0/Actins; 0/Transforming Growth Factor beta; 635-65-4/Bilirubin

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