| Appearance of CAD activity, the rate-limiting enzyme for pyrimidine biosynthesis, as B cells progress into and through the G1 stage of the cell cycle. | |
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MedLine Citation:
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PMID: 7916269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CAD is a multifunctional protein which mediates the first three enzymatic steps of pyrimidine biosynthesis. Previous studies have implicated CAD as a cell cycle regulated protein. In the present paper CAD activity is studied as polyclonally stimulated, murine B cells progress through the early stages of the cell cycle. CAD activity is seen to increase in a biphasic manner. The initial increase in activity occurs prior to or as the cells increase CAD mRNA suggesting that post-translational modification of preformed enzyme may account for at least a portion of this initial enhancement. Increases in CAD mRNA occur by 12 hr poststimulation and precede the second, more dramatic increase in B cell CAD activity. Preliminary experiments failed to provide support of a role for IL-4 in regulating the expression of CAD as B cells progress into G1. CAD enzymatic activity does represent, however, a marker for early B cell cycle progression. |
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Authors:
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G Morford; J N Davidson; E C Snow |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cellular immunology Volume: 158 ISSN: 0008-8749 ISO Abbreviation: Cell. Immunol. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-10-20 Completed Date: 1994-10-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1246405 Medline TA: Cell Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 96-104 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Kentucky Medical Center, Lexington 40536-0084. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartate Carbamoyltransferase / genetics, metabolism* B-Lymphocytes / cytology, enzymology* Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics, metabolism* Cells, Cultured DNA / biosynthesis Dihydroorotase / genetics, metabolism* G1 Phase Male Mice Mice, Inbred DBA Multienzyme Complexes / genetics, metabolism* Pyrimidines / biosynthesis* RNA, Messenger / genetics |
| Grant Support | |
ID/Acronym/Agency:
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NIEHS 5232ES0766/ES/NIEHS NIH HHS; R01 ES05748/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CAD trifunctional enzyme; 0/Multienzyme Complexes; 0/Pyrimidines; 0/RNA, Messenger; 9007-49-2/DNA; EC 2.1.3.2/Aspartate Carbamoyltransferase; EC 3.5.2.3/Dihydroorotase; EC 6.3.5.5/Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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