Document Detail

Apparently non-specific results found using a norovirus antigen immunoassay for fecal specimens from neonates.
MedLine Citation:
PMID:  18165834     Owner:  NLM     Status:  MEDLINE    
Norovirus is increasingly recognized as a frequent cause of non-bacterial gastroenteritis. Despite a 10-fold increase in the number of cases reported following the availability of enzyme immunoassays, there are no reports yet from preterm neonates. We report on a sudden clustering of antigen-positive enzyme immuno assays results in a level III neonatal intensive care unit, involving 22 of 43 infants screened. Although antigen-positive samples were significantly associated with bloody stools (P<0.001) and gastric residues (P<0.02), norovirus infection could not be confirmed by reverse-transcriptase polymerase chain reaction or electron microscopy. We question the validity of the so called norovirus-specific antigen assays and warn against overreacting to positive enzyme immunoassays results without reverse-transcriptase polymerase chain reaction confirmation especially in the neonatal setting.
C Wiechers; A L Bissinger; K Hamprecht; P Kimmig; G Jahn; C F Poets
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of perinatology : official journal of the California Perinatal Association     Volume:  28     ISSN:  0743-8346     ISO Abbreviation:  J Perinatol     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-31     Completed Date:  2008-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8501884     Medline TA:  J Perinatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-81     Citation Subset:  IM    
Department of Neonatology, University Children's Hospital, Tuebingen, Germany.
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MeSH Terms
Caliciviridae Infections / diagnosis*
Enzyme-Linked Immunosorbent Assay / methods,  standards*
False Positive Reactions
Feces / virology*
Gastroenteritis / virology*
Infant, Newborn
Infant, Premature
Intensive Care Units, Neonatal
Intensive Care, Neonatal
Norovirus / isolation & purification*
Reagent Kits, Diagnostic / virology
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Reagent Kits, Diagnostic

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