| Apparent translocation of glucose transport activity in rat epididymal adipocytes by insulin-like effects of high pH or hyperosmolarity. | |
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MedLine Citation:
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PMID: 3003096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The basal and plus insulin states of glucose transport activity in adipocytes are known to show different responses to changes in the pH or osmolarity of the incubation mixture. When the pH was raised from 7 to 8, the basal glucose transport activity (assessed from the rate of 3-O-methyl-D-glucose uptake) was increased approximately 3-fold while the plus insulin activity remained virtually unaffected. Likewise, when cells were exposed to 300 mM sorbitol, the basal glucose transport activity, but not the plus insulin activity, was considerably increased. In both cases, the change in the transport activity was ATP-dependent and was completed in approximately 60 min. The increase in the cellular glucose transport activity was accompanied, in both cases, by an increase in the glucose transport activity in the plasma membrane fraction and a decrease in the activity in the high-speed pellet fraction. The transport activity in the subcellular fractions was determined after reconstitution into egg lecithin liposomes. Both isotonic buffer at pH 8.0 and hypertonic buffer at pH 7.4 significantly stimulated membrane-bound cAMP phosphodiesterase in adipocytes. It is concluded that the above two experimental conditions may induce insulin-like effects in fat cells and may facilitate translocation of the glucose transport activity from an intracellular site to the plasma membrane. |
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Authors:
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N Toyoda; F W Robinson; M M Smith; J E Flanagan; T Kono |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 261 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1986 Feb |
Date Detail:
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Created Date: 1986-03-21 Completed Date: 1986-03-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2117-22 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3',5'-Cyclic-AMP Phosphodiesterases
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metabolism 3-O-Methylglucose Adenosine Triphosphate / pharmacology Adipose Tissue / drug effects, metabolism* Animals Biological Transport, Active / drug effects Epididymis / metabolism Glucose / metabolism* Hydrogen-Ion Concentration Hypertonic Solutions / pharmacology Insulin / pharmacology Kinetics Liposomes / metabolism Male Membrane Proteins / metabolism Methylglucosides / metabolism Monosaccharide Transport Proteins / metabolism* Osmolar Concentration Rats |
| Grant Support | |
ID/Acronym/Agency:
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5R01 AM 06725/AM/NIADDK NIH HHS; 5R01 AM 19925/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hypertonic Solutions; 0/Liposomes; 0/Membrane Proteins; 0/Methylglucosides; 0/Monosaccharide Transport Proteins; 11061-68-0/Insulin; 146-72-5/3-O-Methylglucose; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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