| Apparent dominance of the G1-G3 genetic cluster of Echinococcus granulosus strains in the central inland region of Portugal. | |
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MedLine Citation:
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PMID: 20727984 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Infection by the larval stage of the cestode Echinococcus granulosus causes a disease known as cystic echinococcosis or hydatidosis, which is one of the most widespread zoonotic infections of veterinary and medical importance. Numerous studies have shown that E. granulosus exists as a complex of strains differing in a wide variety of criteria. Ten distinct genotypes (G1-G10) have been identified with a potential impact on the pathology, epidemiology and the effect of the measures implemented for the control of hydatidosis. Our main objective was to carry out a preliminary analysis of the genotypes of E. granulosus circulating in the central inland region of Portugal. Parasite samples (hydatid cysts, n=27) were isolated from the liver and lung of sheep and cattle. The DNA extracted from protoscoleces isolated from the fertile cysts served as a template for the PCR amplification of the part of the mitochondrial cytochrome c oxidase subunit 1 (cox1), ATP synthase F0 subunit 6 (atp6) as well as the large (rrnL/16S) and small (rrnS/12S) ribosomal RNA genes. Similarity searches with homologous sequences in the databanks indicated a very high similarity with references assigned to the G1, G3 and/or G1-G3 complex of Echinococcus strains. Phylogenetic analysis (Bayesian approach) supported these observations, and confirmed the assignment of all the analyzed sequences to the G1-G3 genetic cluster. |
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Authors:
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Sílvia Beato; Ricardo Parreira; Manuela Calado; Maria Amélia A Grácio |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-19 |
Journal Detail:
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Title: Parasitology international Volume: 59 ISSN: 1873-0329 ISO Abbreviation: Parasitol. Int. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9708549 Medline TA: Parasitol Int Country: Netherlands |
Other Details:
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Languages: eng Pagination: 638-42 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Instituto de Higiene e Medicina Tropical (IHMT)/Universidade Nova de Lisboa (UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal. silvia.beato@ihmt.unl.pt |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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