Document Detail


Apparent attachment of Campylobacter and Salmonella to broiler breeder rooster spermatozoa.
MedLine Citation:
PMID:  16615345     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been demonstrated that horizontal and vertical transmission of Salmonella and Campylobacter can occur in broiler breeder flocks. The mechanism of this transmission is still unclear. Previously negative broiler breeder flocks have been reported to become positive with Salmonella, Campylobacter, or both after the introduction of "spike" roosters at 45 wk of age. To determine whether the rooster semen is a possible source of transmission to hens for colonization, we evaluated the association of both Salmonella and Campylobacter spp. to segments (head, midpiece, and tail) of individual spermatozoa after artificial inoculation. Salmonella typhimurium, Salmonella heidelberg, and Salmonella montevideo, or Campylobacter jejuni (in 0.85% saline) was added to a freshly collected (by abdominal massage) aliquot of pooled semen from roosters housed in individual cages. The semen and bacteria solutions were incubated 1 h at room temperature. Samples were fixed using Karnosvsky and Zamboni fixatives for 24 h prior to centrifuging and rinsing in 0.1 M cacodylate x HCl buffer. Individual aliquot samples were then subjected to both scanning (JSM-5800) and transmission (JEM-1210) electron microscopy. The scanning electron microscopy showed that Salmonella was associated with all 3 segments (head, midpiece, and tail) of the spermatozoa and apparently equally distributed. Campylobacter was mainly associated with the midpiece and tail segments; few isolates were located on the head segment. The transmission electron microscopy showed apparent attachment of Salmonella and Campylobacter to the spermatozoa.
Authors:
Y Vizzier-Thaxton; N A Cox; L J Richardson; R J Buhr; C D McDaniel; D E Cosby; J L Wilson; D V Bourassa; M B Ard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  85     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-17     Completed Date:  2006-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  619-24     Citation Subset:  IM    
Affiliation:
Department of Poultry Science, Mississippi State University, Mississippi State 39759, USA. yvizzier@poultry.msstate.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion*
Campylobacter / physiology*,  ultrastructure
Campylobacter Infections / transmission,  veterinary
Chickens / microbiology*
Insemination, Artificial / veterinary
Male
Salmonella / physiology*,  ultrastructure
Salmonella Infections, Animal / transmission
Spermatozoa / microbiology*,  ultrastructure

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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