| Apparatus for measuring rat body volume: a methodological proposition. | |
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MedLine Citation:
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PMID: 17082370 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We propose a communicating-vessels system to measure body volume in live rats through water level detection by hydrostatic weighing. The reproducibility, accuracy, linearity, and reliability of this apparatus were evaluated in two tests using previously weighed water or six aluminum cylinders of known volume after proper system calibration. The applicability of this apparatus to measurement of live animals (Wistar rats) was tested in a transversal experiment with five rats, anesthetized and nonanesthetized. We took 18 measurements of the volume under each condition (anesthetized and nonanesthetized), totaling 90 measurements. The addition of water volumes (50-700 ml) produced a regression equation with a slope of 1.0006 +/- 0.0017, intercept of 0.75 +/- 0.81 (R(2) = 0.99999, standard error of estimate = 0.58 ml), and bias of approximately 1 ml. The differences between cylinders of known volumes and volumes calculated by the system were <0.4 ml. Mean volume errors were 0.01-0.07%. Among the live models, the difference between the volumes obtained for anesthetized and nonanesthetized rats was 0.31 +/- 2.34 (SD) ml (n = 90). These data showed that animal movement does not interfere with the volume measured by the proposed apparatus, and neither anesthesia nor fur shaving is needed for this procedure. Nevertheless, some effort should be taken to eliminate air bubbles trapped in the apparatus or the fur. The proposed apparatus for measuring rat body volume is inexpensive and may be useful for a range of scientific purposes. |
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Authors:
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Rodrigo Hohl; Renato Buscariolli de Oliveira; Denise Vaz de Macedo; René Brenzikofer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-02 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 102 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-07 Completed Date: 2007-04-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1229-34 Citation Subset: IM |
Affiliation:
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Departmento de Bioquímica, Universidade Estadual de Campinas, Campinas, Brasil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Weights and Measures / instrumentation*, methods Male Rats Rats, Wistar Water |
| Chemical | |
Reg. No./Substance:
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7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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