Document Detail


Apoxin I, a novel apoptosis-inducing factor with L-amino acid oxidase activity purified from Western diamondback rattlesnake venom.
MedLine Citation:
PMID:  9083096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Venom of the western diamondback rattlesnake (Crotalus atrox) induces apoptosis in human umbilical vein endothelial cells, which could result in hemorrhage in tissues bitten by the snake. To identify the hemorrhagic factor, we purified a novel protein, apoxin I, from rattlesnake venom. Apoxin I induced apoptosis in human umbilical vein endothelial, human promyelocytic leukemia HL-60, human ovarian carcinoma A2780, and mouse endothelial KN-3 cells. Amino acid sequence analysis of the apoxin I showed close similarity to L-amino acid oxidase from the Malayan pit viper (Calloselasma rhodostoma). The purified apoxin I oxidized L-leucine but not D-leucine to produce H2O2. The apoxin I-induced apoptosis was inhibited by catalase, a H2O2 scavenger. These results indicate that the H2O2 produced by L-amino acid oxidation by apoxin I is involved in the apoxin I-induced apoptosis and in hemorrhage caused by rattlesnake venom.
Authors:
S Torii; M Naito; T Tsuruo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  272     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-08     Completed Date:  1997-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9539-42     Citation Subset:  IM    
Affiliation:
Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / chemistry,  isolation & purification*,  metabolism*
Amino Acid Sequence
Animals
Antioxidants / pharmacology
Apoptosis*
Catalase / metabolism
Chromans / pharmacology
Chromatin / metabolism
Crotalid Venoms / chemistry*,  isolation & purification*,  metabolism
Crotalus
HL-60 Cells
Humans
L-Amino Acid Oxidase
Mice
Molecular Sequence Data
Molecular Weight
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Chromans; 0/Chromatin; 0/Crotalid Venoms; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; EC 1.11.1.6/Catalase; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.2/L-Amino Acid Oxidase

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