Document Detail


Apoptotic, necrotic, or fused tumor cells: an equivalent source of antigen for dendritic cell loading.
MedLine Citation:
PMID:  16738802     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The identification of the most efficient strategy for tumor antigen loading of dendritic cells (DCs) remains a challenge in cancer immunotherapy protocols. Autologous dead tumor cells have been demonstrated to constitute an acceptable source of multiple tumor-associated antigens (TAA) to pulse DCs. However the optimal approach for inducing cell death that would lead to effective endocytosis and activation of DCs remains controversial. In this study we have induced and defined 3 distinct mechanisms of tumor cell death (apoptosis, necrosis and fusion-mediated cell death), and investigated their differential effects on DCs. Bone marrow-derived DCs demonstrated comparable uptake of primary apoptotic, necrotic, or fused dead tumor cells. Furthermore, the distinct modes of cancer cell death had analogous potential in activating the transcription factors NF-kappaB and STAT1 and in maturing DCs, resulting in an equally effective stimulation of immune T cells. The current study therefore provides further informations on the use of dead whole tumor cells as antigen sources for effective active anti-cancer immunotherapy.
Authors:
Nicolas Larmonier; Delphine Mérino; Alexandra Nicolas; Dominique Cathelin; Angélique Besson; Andrew Bateman; Eric Solary; François Martin; Emmanuel Katsanis; Bernard Bonnotte
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  11     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-20     Completed Date:  2006-12-14     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1513-24     Citation Subset:  IM    
Affiliation:
INSERM U517, IFR 100, Faculty of Medicine and Pharmacy, 7 Boulevard Jeanne d'Arc, 21079 Dijon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation / immunology*
Apoptosis / immunology*
Cell Fusion*
Colonic Neoplasms / immunology
Dendritic Cells / immunology*
Interleukin-12 / biosynthesis
Lymphocyte Activation
NF-kappa B / metabolism
Necrosis / immunology*
Phenotype
Rats
STAT1 Transcription Factor / metabolism
T-Cell Antigen Receptor Specificity
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
R01 CA104926/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/NF-kappa B; 0/STAT1 Transcription Factor; 187348-17-0/Interleukin-12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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