| Apoptotic effect of ganciclovir on primary effusion lymphoma cells infected with Kaposi's sarcoma-associated herpesvirus. | |
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MedLine Citation:
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PMID: 16838851 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We evaluated the cytotoxic and apoptotic effects of two purine nucleoside analogues, acyclovir (ACV) and ganciclovir (GCV), on lymphoma cells stably harboring Kaposi's sarcoma-associated herpesvirus (KSHV). Colorimetric caspase assay, flow cytometry, and immunoblotting with antibodies against apoptosis-related molecules revealed that GCV has cytotoxic activity toward KSHV-infected primary effusion lymphoma cells, while ACV has weak or little activity. In addition to the GCV-induced cytotoxicity, apoptosis via caspase-7/8, cleavage of poly(ADP-ribose) polymerase, and accumulation of p53 and p21 were induced by GCV treatment. In contrast, neither ACV nor GCV have cytotoxicity- or apoptosis-inducing activities toward uninfected cells. |
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Authors:
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Masahiro Fujimuro; Hisanori Inoue; Yasuhiro Teishikata; Yuriko Ishida; Hideyoshi Yokosawa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleosides, nucleotides & nucleic acids Volume: 25 ISSN: 1525-7770 ISO Abbreviation: Nucleosides Nucleotides Nucleic Acids Publication Date: 2006 |
Date Detail:
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Created Date: 2006-07-14 Completed Date: 2006-11-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892832 Medline TA: Nucleosides Nucleotides Nucleic Acids Country: United States |
Other Details:
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Languages: eng Pagination: 635-45 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan fuji2@pharm.hokudai.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyclovir
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pharmacology Apoptosis / drug effects* Caspase 3 / metabolism Caspase 7 / metabolism Ganciclovir / pharmacology*, therapeutic use Herpesvirus 8, Human / physiology* Humans Lymphoma / drug therapy*, pathology, virology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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59277-89-3/Acyclovir; 82410-32-0/Ganciclovir; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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