Document Detail


Apoptotic effect of alpha-mangostin on head and neck squamous carcinoma cells.
MedLine Citation:
PMID:  21106189     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: The purposes of this study were to measure the cytotoxic effect of alpha-mangostin on head and neck squamous cell carcinoma (HNSCC) cell lines, to evaluate the apoptotic aspect of dead cells, and to identify the molecular mechanism involved in apoptosis.
METHODS: The human HNSCC cell lines HN-22, HN-30 and HN-31 were treated with alpha-mangostin. The apoptotic effects of alpha-mangostin on HNSCC cells were determined by observation the morphological changes of cells, immunofluorescence for single-stranded DNA (ssDNA), and DNA fragmentation analysis. The expression of bax, bcl-2, and p53 were detected by RT-PCR and Western blot analysis.
RESULTS: Alpha-mangostin showed excellent apoptotic effects on HNSCC cell lines, which induced the down-regulation of bcl-2, but up-regulation of bax and p53 in HN-22, HN-30 and HN-31.
CONCLUSION: The present study suggests that the induction of apoptosis by alpha-mangostin seemed to be modulated by bcl-2, bax and p53 level in HNSCC cell lines.
Authors:
Ruchadaporn Kaomongkolgit; Niratcha Chaisomboon; Prasit Pavasant
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Publication Detail:
Type:  Journal Article     Date:  2010-11-23
Journal Detail:
Title:  Archives of oral biology     Volume:  56     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  483-90     Citation Subset:  D; IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok-Nakornsawan Rd, Mueng, Phitsanulok 65000, Thailand.
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