Document Detail

Apoptotic effect of Polygonum Cuspidatum in oral cancer cells through the regulation of specificity protein 1.
MedLine Citation:
PMID:  20659264     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVES:   The aim of this study was to evaluate the growth inhibitory and apoptosis-inducing effects and mechanisms of Polygonum cuspidatum root in oral cancer cells.
MATERIALS AND METHODS:   The testing materials were separated by normal-phase silica gel liquid chromatography. The effect of P. cuspidatum root on apoptotsis and its mechanism were performed using 3-(4,5-dimethylthiazol-20yl)-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium) (MTS) assay, western blot analysis, RT-PCR, promoter assay, and (4'-6-Diamidino-2-phenylindole) (DAPI) staining .
RESULTS:   The methanol extract of P. cuspidatum (MEPC) inhibited the proliferation of oral cancer cells by inducing caspase-dependent apoptosis. Protein and mRNA expression levels and the transactivation of Specificity protein 1 (Sp1) were markedly decreased in KB cells treated with MEPC. Ethyl acetate fraction (EA) from MEPC was more potent than aqueous fraction (AQ) from MEPC to induce apoptosis. F2, F3, and F4 from EA differentially inhibited the growth of KB cells, and it depends on the amount of Emodin in F2, F3, and F4. Moreover, Emodin inhibited oral cancer cell growth and induced caspase-dependent apoptosis by decreasing Sp1. MEPC also decreased an apoptosis-related downstream target of Sp1 protein, survivin.
CONCLUSION:   The results from this study strongly suggest that MEPC, its fraction, and Emodin may be potential bioactive materials to cause apoptosis mechanism via the down-regulation of Sp1 in oral cancer cells.
J-A Shin; J-H Shim; J-G Jeon; K-H Choi; E-S Choi; N-P Cho; S-D Cho
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oral diseases     Volume:  17     ISSN:  1601-0825     ISO Abbreviation:  Oral Dis     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508565     Medline TA:  Oral Dis     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  162-70     Citation Subset:  D    
Copyright Information:
© 2010 John Wiley & Sons A/S.
Department of Oral Pathology, School of Dentistry and Institute of Oral Bioscience, BK21 project, Chonbuk National University, Jeonju Department of Preventive Dentistry, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju, Korea Laboratory for Oral disease-related Compounds, School of Dentistry, Chonbuk National University, Jeonju, Korea.
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