Document Detail


Apoptotic cell death induced by Acinetobacter baumannii in epithelial cells through caspase-3 activation.
MedLine Citation:
PMID:  11890571     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epithelial cell death induced by Acinetobacter baumannii infection was investigated using in vitro assays. Eight hours after live A. baumannii infection, HeLa cells exhibited detachment from the dish, rounding morphologies, high proportions of trypan blue-positive cells and extensive DNA breakdown with faint apoptotic banding, which is indicative of cells undergoing apoptosis. The enzymatic activity of caspase-3 was increased in cells as early as 2 h after infection. In addition, apoptosis of HeLa cells was induced by treatment with bacterial culture filtrates but not with formalin-killed bacteria. These results indicate that A. baumannii infection triggers apoptosis in HeLa cells through caspase-3 activation.
Authors:
J C Lee; J Y Oh; K S Kim; Y W Jeong; J C Park; J W Cho
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  109     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2002-03-13     Completed Date:  2002-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  679-84     Citation Subset:  IM    
Affiliation:
Department of Microbiology, College of Medicine, Seonam University, Namwon, Chunpook, Korea. leejc@tiger.seonam.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Acinetobacter*
Acinetobacter Infections / pathology*,  physiopathology
Apoptosis / physiology*
Caspase 3
Caspases / physiology*
Enzyme Activation / physiology
Epithelial Cells / microbiology*,  pathology*,  physiology
Hela Cells
Humans
Signal Transduction / physiology*
Chemical
Reg. No./Substance:
EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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