Document Detail

Apoptotic cell death of high polyploid cells in a cultured sarcoma cell line.
MedLine Citation:
PMID:  9855116     Owner:  NLM     Status:  MEDLINE    
It is well known that DNA-ploidy is useful independent prognosticator of malignancy. However, the biological significance of polyploid cells and the relation between polyploidy and prognosis is not well understood. We analyzed DNA ploidy by flow cytometry in Meth-A cells (a cultured sarcoma cell line) after treatment with K252a, a protein kinase inhibitor, and showed induction of polyploidization. Apoptotic cell death of the high polyploid cells was verified by flow cytometry, morphological observation and gel analysis of DNA integrity. Expression of tumor-suppressor nuclear protein p53 investigated by immunohistochemistry was increased 10-fold or more in cells with 16C (C = haploid DNA content) relative to cells with 2C, suggesting that the overexpression of p53 was involved in the apoptosis. These results may be of clinical relevance since it has been known that both DNA ploidy and p53 expression have prognostic significance.
Z P Zong; K Fujikawa-Yamamoto; T Ota; M Murakami; A L Li; N Yamaguchi; M Tanino; S Odashima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell structure and function     Volume:  23     ISSN:  0386-7196     ISO Abbreviation:  Cell Struct. Funct.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1999-02-02     Completed Date:  1999-02-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7608465     Medline TA:  Cell Struct Funct     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  231-7     Citation Subset:  IM    
Division of Basic Science, Medical Research Institute, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
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MeSH Terms
Carbazoles / pharmacology
Cell Division / drug effects
Cell Survival / drug effects
DNA, Neoplasm / analysis*
Enzyme Inhibitors / pharmacology
Flow Cytometry
Indole Alkaloids
Mice, Inbred BALB C
Protein Kinase C / antagonists & inhibitors
Sarcoma, Experimental / drug therapy,  genetics,  metabolism,  pathology*
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / metabolism
Reg. No./Substance:
0/Carbazoles; 0/DNA, Neoplasm; 0/Enzyme Inhibitors; 0/Indole Alkaloids; 0/Tumor Suppressor Protein p53; 56-49-5/Methylcholanthrene; 97161-97-2/K 252; EC Kinase C

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