Document Detail


Apoptotic cell clearance in systemic lupus erythematosus. II. Role of beta2-glycoprotein I.
MedLine Citation:
PMID:  9485079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To analyze the contribution of beta2-glycoprotein I (beta2-GPI) to apoptotic cell recognition by antiphospholipid antibodies (aPL) and macrophages from patients with autoimmune disease. METHODS: Phosphatidylserine expression by Jurkat cells undergoing apoptosis upon CD95 crosslinking or ultraviolet irradiation was verified by confocal microscopy of cells stained with fluorescein isothiocyanate-labeled annexin V. Beta2-GPI was purified by heparin/cationic-exchange chromatography and was biotinylated or used to purify beta2-GPI-specific antibodies by affinity chromatography. Binding to apoptotic cells was assessed by flow cytometry. The clearance of 3H-labeled, apoptotic cells by macrophages was assessed by beta counting. RESULTS: The array of epitopes generated by beta2-GPI association with apoptotic cells specifically targets their recognition and is required for their opsonization by human aPL. Nevertheless, beta2-GPI is not required for apoptotic cell clearance by human macrophages in the absence of aPL. CONCLUSION: The proinflammatory clearance of aPL-opsonized apoptotic cells, but not the nonphlogistic clearance of apoptotic cells by scavenger macrophages, depends on beta2-GPI.
Authors:
A A Manfredi; P Rovere; S Heltai; G Galati; G Nebbia; A Tincani; G Balestrieri; M G Sabbadini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  41     ISSN:  0004-3591     ISO Abbreviation:  Arthritis Rheum.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-19     Completed Date:  1998-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  215-23     Citation Subset:  AIM; IM    
Affiliation:
Istituto Scientifico H. S. Raffaele, and Università di Milano, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Antiphospholipid / immunology
Apoptosis / physiology*
Glycoproteins / metabolism,  physiology*
Humans
Jurkat Cells
Lupus Erythematosus, Systemic / metabolism,  pathology*,  physiopathology*
Macrophages / physiology
Opsonin Proteins / immunology
Phosphatidylserines / metabolism
beta 2-Glycoprotein I
Chemical
Reg. No./Substance:
0/Antibodies, Antiphospholipid; 0/Glycoproteins; 0/Opsonin Proteins; 0/Phosphatidylserines; 0/beta 2-Glycoprotein I

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