| Apoptotic and autophagic responses to photodynamic therapy in 1c1c7 murine hepatoma cells. | |
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MedLine Citation:
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PMID: 21555918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photodynamic therapy (PDT) is a process that can induce apoptosis, autophagy or both depending on the cell phenotype. Apoptosis is a pathway to cell death while autophagy can protect from photokilling or act as a death pathway. In a previous study, we reported a cytoprotective effect of autophagy in murine leukemia cell lines where both autophagy and apoptosis occur within minutes after irradiation of photosensitized cells. In this study, we examined the effects of mitochondrial photodamage catalyzed by low (≤ 1 μM) concentrations of the photosensitizing agent termed benzoporphyrin derivative (BPD, Verteporfin) on murine hepatoma 1c1c7 cells. Apoptosis was not observed until several hours after irradiation of photosensitized cells. Autophagy was clearly cytoprotective since PDT efficacy was significantly enhanced in a knockdown sub-line (KD) in which the level of a critical autophagy protein (Atg7) was markedly reduced. This result indicates that autophagy can protect from phototoxicity even when apoptosis is substantially delayed. Much higher concentrations (≥ 10 μM) of BPD had previously been shown to inhibit autophagosome formation. Phototoxicity studies performed with 10 μM BPD and a proportionally reduced light dose were consistent with the absence of an autophagic process in wild-type (WT) cells under these conditions. |
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Authors:
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Michelle Andrzejak; Michael Price; David H Kessel |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-09-01 |
Journal Detail:
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Title: Autophagy Volume: 7 ISSN: 1554-8635 ISO Abbreviation: Autophagy Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-30 Completed Date: 2011-12-28 Revised Date: 2012-02-08 |
Medline Journal Info:
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Nlm Unique ID: 101265188 Medline TA: Autophagy Country: United States |
Other Details:
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Languages: eng Pagination: 979-84 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Detroit Mercy, Detroit, MI, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* / drug effects, radiation effects Autophagy* / drug effects, radiation effects Carcinoma, Hepatocellular / drug therapy*, pathology*, ultrastructure Cell Line, Tumor Cell Shape / drug effects, radiation effects Cell Survival / drug effects, radiation effects Dermatitis, Phototoxic Dose-Response Relationship, Drug Gene Knockdown Techniques Leukemia / pathology Light Liver Neoplasms / drug therapy*, pathology*, ultrastructure Mice Microtubule-Associated Proteins / metabolism Photochemotherapy* Photosensitizing Agents / pharmacology, therapeutic use Porphyrins / pharmacology, therapeutic use Vacuoles / drug effects, radiation effects, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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CA 23378/CA/NCI NIH HHS; GM-058905-11/GM/NIGMS NIH HHS; R01 CA023378-30/CA/NCI NIH HHS; R01 CA023378-31/CA/NCI NIH HHS; T32-CA009531/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Atg7 protein, mouse; 0/Microtubule-Associated Proteins; 0/Photosensitizing Agents; 0/Porphyrins; 129497-78-5/verteporfin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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