| Apoptotic activity of frog Bombina maxima skin albumin. | |
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MedLine Citation:
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PMID: 16343964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Albumin, the most abundant protein components of blood plasma, is synthesized and secreted by liver cells in vertebrates. Recently, it was demonstrated that frog Bombina maxima albumin is also expressed in skin. Both B. maxima albumins from skin and serum (BmA-skin and BmA-serum) have similar biochemical characteristics except that the former contains haem b. Present studies showed that BmA-skin exhibited cytotoxic activity on H9 and C8166 cells. Pretreated with hemin to induce erythroid differentiation, K562 cells lost their resistance to cytotoxicity of BmA-skin. After treating cells with BmA-skin for 48 h, 50 percentage cytotoxic concentrations (CC(50)) of BmA-skin on H9, C8166 and hemin-treated K562 cells were 1.31+/-0.09, 1.59+/-0.08 and 2.28+/-0.06 microM, respectively. The cell death induced by BmA-skin was mediated by apoptosis of the tested cell lines, as demonstrated by nuclear morphological changes, DNA fragmentation and DNA hypodiploidy of apoptosis cells. At BmA-skin concentration of 2 microM, 27.3%, 19.7% and 17.8% of H9, C8166 and hemin-treated K562 cells were found to be apoptotic. In contrast, BmA-serum possessed no cytotoxic and apoptosis-inducing activity on all the cell lines tested, even with concentration used up to 15 microM. These results indicated that bound haem b in BmA-skin contributed significantly to its cytotoxic and apoptosis-inducing activity on the cell lines assayed. |
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Authors:
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Ying-Xia Zhang; Yuan-Yuan Wang; Wen-Hui Lee; Yong-Tang Zheng; Yun Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-12-15 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: 143 ISSN: 1096-4959 ISO Abbreviation: Comp. Biochem. Physiol. B, Biochem. Mol. Biol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-03 Completed Date: 2006-06-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 153-9 Citation Subset: IM |
Affiliation:
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Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China. zhangy@mail.kiz.ac.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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pharmacology* Animals Anura* Apoptosis / drug effects* Cell Death / drug effects Cell Line Cytotoxins / pharmacology* Dose-Response Relationship, Drug Humans Skin / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Cytotoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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