| Apoptotic signalling in HeLa Hep2 cells following 5 Gy of cobalt-60 gamma radiation. | |
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MedLine Citation:
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PMID: 20032379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The apoptotic signalling pathways involved in the delayed type of apoptosis occurring in HeLa Hep2 cells following radiation were investigated. MATERIALS AND METHODS: HeLa Hep2 cells were exposed to 5 Gy of cobalt-60 radiation. The activation of caspase-2, caspase-8, caspase-9 and effector caspase-3 was investigated by caspase assay plates and Western blots. Cleavage of poly (ADP-ribose) polymerase (PARP) was analysed on Western blots. HeLa Hep2 cells were irradiated with or without preincubation with inhibitors of protein synthesis (cycloheximide, CHX) and caspases, followed by TUNEL staining and caspase assay plate evaluation. RESULTS: Initiator caspases-2, -8, -9, and effector caspase-3, were found to be activated and PARP cleaved following irradiation. CHX completely inhibited the caspase activation and the associated apoptosis. Pretreatment with caspase-2 inhibitor indicated that caspase-2 was involved in the execution of the apoptosis. CONCLUSION: Activation of the apoptotic signalling pathways following irradiation of HeLa Hep2 cells includes components from the intrinsic as well as the extrinsic pathways and seems to require de novo protein synthesis. |
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Authors:
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David Eriksson; Per-Olov Löfroth; Lennart Johansson; Katrine Riklund; Torgny Stigbrand |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 29 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-12-24 Completed Date: 2010-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 4361-6 Citation Subset: IM |
Affiliation:
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Department of Immunology, Umeå University, SE-90185 Umeå, Sweden. david.eriksson@climi.umu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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enzymology,
pathology*,
radiotherapy Apoptosis / drug effects, physiology, radiation effects* Caspase 2 / antagonists & inhibitors, metabolism Caspases / antagonists & inhibitors, metabolism Cell Line, Tumor Cobalt Radioisotopes Enzyme Activation / radiation effects Female Gamma Rays Hela Cells Humans Isoenzymes / antagonists & inhibitors, metabolism Poly(ADP-ribose) Polymerases / metabolism Signal Transduction / radiation effects Uterine Cervical Neoplasms / enzymology, pathology*, radiotherapy |
| Chemical | |
Reg. No./Substance:
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0/Cobalt Radioisotopes; 0/Isoenzymes; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 2; EC 3.4.22.-/Caspases |
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