| Apoptotic Cell Death in the Fission Yeast Schizosaccharomyces pombe Induced by Valproic Acid and Its Extreme Susceptibility to pH Change. | |
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MedLine Citation:
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PMID: 21670521 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Schizosaccharomyces pombe treated with valproic acid died with apoptotic markers such as DNA fragmentation, loss of a mitochondrial electrochemial gradient and chromatin condensation, independently of metacaspase, a yeast homolog of metazoan caspase. Sensitivity to valproic acid was strongly dependent on growth phase. Cells in a later growth phase were much more sensitive to valproic acid than those in an earlier one. Altering the pH of the medium with HCl and with NaOH also caused remarkable changes in sensitivity. Cells in an acidic medium were more sensitive to valproic acid. This pH-dependent change in sensitivity did not require de novo protein synthesis, and a change in pH 60 min after the administration of valproic acid affected sensitivity. These results suggest that the intracellular cell death process was susceptible to extracellular pH. Although a sir2 mutant of Saccharomyces cerevisiae has been reported to be resistant to valproic acid, mutations in sir2 did not affect the sensitivity to valproic acid of S. pombe. |
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Authors:
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Norihiro Mutoh; Satoko Kitajima; Shigeyuki Ichihara |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-13 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: - ISSN: 1347-6947 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Genetics, Institute for Developmental Research, Aichi Human Service Center. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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