Document Detail

Apoptosis removes chick embryo tail gut and remnant of the primitive streak.
MedLine Citation:
PMID:  8725288     Owner:  NLM     Status:  MEDLINE    
Removal of transient features in morphogenesis of chick embryo tail is by programmed cell death. We used ApopTagTM (Oncor, Gaithersburg, MD) with the peroxidase/diaminobenzidine (DAB) procedure to correlate apoptosis with earlier reports of patterns of cell death in stage HH17-25 embryos, and our results suggest that the cell death inferred with supravital staining and appearance of cells in morphogenesis of the tail bud is programmed cell death called apoptosis. Apoptosis markers in tail bud are most abundant in the median cell cord of occluded degenerating tail gut. Tail bud mesenchyme marks for apoptosis most frequently in the ventrum of older stages, where cell death has been reported. Cells of the remnant of the primitive streak (Hensen's node) mark for apoptosis, suggesting that programmed cell death is a stop signal for axial organization at the caudal terminus. Apoptosis markers in postmembrane cloacal endoderm anticipate the transient cloacal fenestra. Lack of apoptosis markers in neural tube, notochord, and somites supports the suggestion of Schoenwolf ([1981] Anat, Embryol. (Beri.) 162:183-197) that cells of those areas in the tail bud are assimilated into the growing rump of the chick embryo. Lack of markers in neural tube of tail bud formed by secondary neurulation suggests that apoptosis is not involved in cavitation of medullary cord, but further investigation is necessary. A limited investigation of pharyngeal membranes and midgut, where cell death has not been reported to be as important in morphogenesis, did not show apoptosis markers in those tissues (Miller and Briglin [1994] "Cell Death in Development and Cancer," Houston: University of Texas MD Anderson Cancer Center, pp, 82-83). Absence of apoptosis markers in roof of gut tube suggests that the lower frequency of thymidine labeling reported for those cells (Miller [1986] Anat. Rec. 214: 87A) is not a result of apoptosis. Clearly marked cells correlated with expected locations of migrating neural crest and primordial germ cells in these stages, but distribution of apoptosis markers was not abundant or general for either cell type.
S A Miller; A Briglin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  206     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-11-04     Completed Date:  1996-11-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  212-8     Citation Subset:  IM    
Department of Biology, Hamilton College, Clinton, New York 13323, USA.
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MeSH Terms
Apoptosis / physiology*
Bursa of Fabricius / cytology,  embryology
Chick Embryo
Cloaca / cytology,  embryology
Digestive System / cytology,  embryology
Gastrula / cytology*
Notochord / cytology,  embryology
Pharynx / cytology,  embryology
Somites / cytology
Tail / cytology,  embryology*

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