Document Detail


Apoptosis rate can be accelerated or decelerated by overexpression or reduction of the level of elongation factor-1 alpha.
MedLine Citation:
PMID:  9457069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peptide chain elongation factor-1 alpha (EF-1 alpha) is required for the binding of aminoacyl-tRNAs to acceptor sites of ribosomes during protein synthesis. More recently, EF-1 alpha has been shown to be involved in cytoskeletal organization. The elongation factor functions in actin bundling and microtubule severing. Moreover, it can activate the phosphatidylinositol-4 kinase whose substrates are involved in regulation of actin polymerization. The expression level of EF-1 alpha is regulated in many situations such as growth arrest, transformation, and aging. Because of this regulation of EF-1 alpha in various states of cell life, and its key position in protein synthesis as well as cytoskeletal organization, we chose to investigate the effect of its expression levels on apoptosis. Apoptosis is a complex event regulated through numerous activators and inhibitors. In some situations, protein synthesis is required for apoptosis to be triggered. Investigation of the effect of altered levels of elongation factor-1 alpha on apoptosis is of particular interest since it may affect both protein synthesis and cytoskeletal organization. For example, reduction of EF-1 alpha leads to a reduced protein synthesis rate, which might reduce the presence of those "killer factors" triggering apoptosis. EF-1 alpha involvement in cytoskeletal organization is another example, since cytoskeletal organization undergoes dramatic changes during apoptosis. Thus, this study has been planned to ascertain whether hypo- and hyperexpression of EF-1 alpha protein, achieved by constructing expression vectors with the EF-1 alpha cDNA in its antisense or sense orientation under the control of a cytomegalovirus promoter, can produce stable transfectants with either heightened or reduced responsiveness to apoptosis stimuli. Our results show the following: (1) induction of apoptosis by serum deprivation shows that antisense EF-1 alpha provides cells significant protection from apoptotic cell death and (2) EF-1 alpha overexpression causes a faster rate of cell death. These findings suggest that when EF-1 alpha protein is abundant the cells are proapoptosis, and vice versa in low abundance the cells are in the mode of antiapoptosis. Therefore, changes in levels of EF-1 alpha may be one of the global pivotal regulators modulating the rate of apoptosis.
Authors:
A Duttaroy; D Bourbeau; X L Wang; E Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  238     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-23     Completed Date:  1998-02-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  168-76     Citation Subset:  IM    
Affiliation:
Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Apoptosis / physiology*
Cell Survival
Clone Cells
Cycloheximide / pharmacology
DNA Fragmentation
DNA, Antisense
Gene Expression Regulation*
Kinetics
Mice
Peptide Elongation Factor 1
Peptide Elongation Factors / biosynthesis*
Transfection
Chemical
Reg. No./Substance:
0/DNA, Antisense; 0/Peptide Elongation Factor 1; 0/Peptide Elongation Factors; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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