Document Detail


Apoptosis and necrosis induced by sulfur mustard in Hela cells.
MedLine Citation:
PMID:  10678094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the apoptotic effect of sulfur mustard (SM) on Hela cells. METHODS: Exponentially growing Hela cells were treated with SM at various concentrations for 3 h, then apoptosis was examined by electron-microscope, DNA gel electrophoresis, and flow cytometry. RESULTS: SM 1 mumol.L-1 arrested cell growth. After treatment with SM 10-100 mumol.L-1, cells were mainly blocked at G1-phase with apoptosis. Agarose gel electrophoresis of DNA from cells treated with SM revealed "DNA Ladder." About 33% of the Hela cells showed apoptosis 12 h after 3-h treatment with SM 100 mumol.L-1 as determined by flow cytometry and the S-phase cells were more susceptible. However, SM 1000 mumol.L-1 caused marked necrosis in Hela cells. CONCLUSION: SM caused 2 distinct forms of cell death, apoptosis or necrosis, in Hela cells in a concentration-dependent manner.
Authors:
J Sun; Y X Wang; M J Sun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zhongguo yao li xue bao = Acta pharmacologica Sinica     Volume:  20     ISSN:  0253-9756     ISO Abbreviation:  Zhongguo Yao Li Xue Bao     Publication Date:  1999 May 
Date Detail:
Created Date:  2000-05-18     Completed Date:  2000-05-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8100330     Medline TA:  Zhongguo Yao Li Xue Bao     Country:  CHINA    
Other Details:
Languages:  eng     Pagination:  445-8     Citation Subset:  IM    
Affiliation:
Department of Biochemical Pharmacology, Academy of Military Medical Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Cell Cycle
Hela Cells
Humans
Mustard Gas / pharmacology*
Necrosis
Chemical
Reg. No./Substance:
505-60-2/Mustard Gas

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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