| Apoptosis and necrosis in senescent human fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 16803991 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To study the role of cell death in the aging process, cell death during spontaneous cellular senescence in vitro was examined with normal human fibroblasts. A small subset of the senescent cells showed aberrant morphology such as remarkable nuclear fragmentation or multiple micronuclei, and such cells often showed positive reactions with antibody to phosphorylated pRb. Cells showing caspase activation and binding of Annexin V, which indicate apoptotic change, increased in the senescent phase in flow cytometry analysis. Propidium iodide-positive cells, however, also increased with passaging. The results suggest that both apoptosis and necrosis are involved in cell death of senescent human fibroblasts. |
| | |
Authors:
|
Susumu Ohshima |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Annals of the New York Academy of Sciences Volume: 1067 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 2006 May |
Date Detail:
|
Created Date: 2006-06-28 Completed Date: 2006-07-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 228-34 Citation Subset: IM |
Affiliation:
|
Division of Morphological Science, Biomedical Research Center, Saitama Medical School, Morohongo, Iruma, Japan. sohshima@saitama-med.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Annexin A5
/
metabolism Apoptosis* Caspases / antagonists & inhibitors, metabolism Cell Aging* Cell Nucleus / metabolism Cell Shape Cells, Cultured Enzyme Activation / drug effects Fibroblasts / cytology, physiology* Humans Necrosis / pathology Trypsin / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Annexin A5; EC 3.4.21.4/Trypsin; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proteasomal oscillation during mild heat shock in aging human skin fibroblasts.
Next Document: Aging of murine mesenchymal stem cells.