Document Detail

Apoptosis of mouse embryonic stem cells induced by single cell suspension.
MedLine Citation:
PMID:  10798319     Owner:  NLM     Status:  MEDLINE    
Embryonic stem cells (ES cells) are pluripotential, and are therefore used to construct gene knock-out mice. We found that the apoptosis of mouse ES cells was induced when the cells were dispersed as single cells, whereas this process was suppressed when they proliferated in aggregates. The apoptosis of ES cells was repressed when the cells were cultured on feeders prepared from STO cells, a cell line established from embryonic fibroblasts. Culture supernatants from STO cells did not block the apoptosis of ES cells, which suggests that a direct interaction between ES cells and STO cells is required for the suppression of apoptosis. The viability of ES cells examined by the trypan blue exclusion test or by the MTT ((3-4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction assay decreased dramatically when the cells were dispersed in phosphate-buffered saline PBS. Cellular activity was restored by the addition of culture medium for ES cells. Glucose in the medium was found to be a major factor responsible for the restoration. Amino acids also restored the decrease in reduction of MTT. Suspension of the ES cells in PBS(-) caused leakage of the nucleosome into cytoplasm. Results indicate that the single cell suspension of ES cells leads to leakage of substrates for oxidative phosphorylation from the mitochondria, and that these cells finally become committed to apoptosis.
R Koyanagi-Katsuta; N Akimitsu; N Arimitsu; T Hatano; K Sekimizu
Related Documents :
19041749 - Motor deficit in a drosophila model of mucolipidosis type iv due to defective clearance...
8479619 - Apoptosis in the anterior pituitary gland of the rat: studies with estrogen and bromocr...
15808579 - Mizoribine-mediated apoptotic signaling pathway in human t-cell line.
11445669 - Quantitative determination of apoptosis on leukemia cells by infrared spectroscopy.
9149129 - Sulindac sulfide inhibits the proliferation of colon cancer cells: diminished expressio...
10706479 - Patterns of cell death induced by eluates from denture base acrylic resins in u-937 hum...
1563479 - Cell cycle-dependent vimentin expression in elutriator-synchronized, tpa-treated mpc-11...
11741589 - Proliferation of c6 glioma cells is blunted by the increase in gap junction communicati...
24681909 - Flowfit: a bioconductor package to estimate proliferation in cell-tracking dye studies.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue & cell     Volume:  32     ISSN:  0040-8166     ISO Abbreviation:  Tissue Cell     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-07-07     Completed Date:  2000-07-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0214745     Medline TA:  Tissue Cell     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  66-70     Citation Subset:  IM    
Department of Medico-Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / pharmacology
Apoptosis / drug effects,  physiology*
Cell Communication
Cell Line
Cell Survival / drug effects
Culture Media
Cytological Techniques
Edetic Acid / pharmacology
Glucose / pharmacology
Nucleosomes / metabolism
Trypsin / pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Nucleosomes; 50-99-7/Glucose; 60-00-4/Edetic Acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An immunohistochemical study of the insulin-, glucagon- and somatostatin-immunoreactive cells in the...
Next Document:  The Harderian gland of the Dhub lizard Uromastyx microlepis of the Kuwaiti desert: an ultrastructura...