Document Detail

Apoptosis: molecular regulation of cell death and hematologic malignancies.
MedLine Citation:
PMID:  11936259     Owner:  NLM     Status:  MEDLINE    
We describe the molecular mechanisms of apoptosis and its relationships with hematologic malignancies, stressing the concept that, both positive and negative deregulation of apoptosis, may be involved in hematologic human diseases. So, this fundamental process must be balanced by so far unknown mechanisms, involving caspases (cysteine proteases, cleaving the protein substrate after an aspartate residue). These, so far known, ten proteases, are interconnected in a molecular cascade, initiated by the release of cytochrome C from mitochondrial membranes and its interaction with APAF-1 (the homolog of the Caenorhabditis e. CED-4) and with caspase 9, that initiates the proteolitic cascade (1,2). The conclusion is that apoptosis is a very important process, but yet poorly known in molecular details, in spite of the efforts of many scientists. Even the role of bcl-2, the main gene protecting from apoptosis, is still unknown. We close this chapter with a list of ten different technical approaches that can be useful tools to study apoptosis, and tracing the molecular principles on which they are based.
Vincenzo Chiarugi; Marina Cinelli; Lucia Magnelli; Sbarba Persio Dello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Molecular biotechnology     Volume:  20     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-08     Completed Date:  2002-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-14     Citation Subset:  IM    
Dept. of Experimental Pathology and Oncology. Universita degli Studi di Firenze, Italy.
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MeSH Terms
Antibodies, Monoclonal / metabolism
Apoptotic Protease-Activating Factor 1
Cell Death
DNA Damage
Flow Cytometry
Hematologic Neoplasms / pathology*
In Situ Nick-End Labeling
Proteins / metabolism*
Reg. No./Substance:
0/APAF1 protein, human; 0/Antibodies, Monoclonal; 0/Apoptotic Protease-Activating Factor 1; 0/Proteins

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