Document Detail


Apoptosis: molecular regulation of cell death and hematologic malignancies.
MedLine Citation:
PMID:  11936259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe the molecular mechanisms of apoptosis and its relationships with hematologic malignancies, stressing the concept that, both positive and negative deregulation of apoptosis, may be involved in hematologic human diseases. So, this fundamental process must be balanced by so far unknown mechanisms, involving caspases (cysteine proteases, cleaving the protein substrate after an aspartate residue). These, so far known, ten proteases, are interconnected in a molecular cascade, initiated by the release of cytochrome C from mitochondrial membranes and its interaction with APAF-1 (the homolog of the Caenorhabditis e. CED-4) and with caspase 9, that initiates the proteolitic cascade (1,2). The conclusion is that apoptosis is a very important process, but yet poorly known in molecular details, in spite of the efforts of many scientists. Even the role of bcl-2, the main gene protecting from apoptosis, is still unknown. We close this chapter with a list of ten different technical approaches that can be useful tools to study apoptosis, and tracing the molecular principles on which they are based.
Authors:
Vincenzo Chiarugi; Marina Cinelli; Lucia Magnelli; Sbarba Persio Dello
Related Documents :
9190889 - Affinity labeling displays the stepwise activation of ice-related proteases by fas, sta...
20944749 - Pannexin 1 channels mediate 'find-me' signal release and membrane permeability during a...
12053139 - Mechanisms of cell death in the injured auditory system: otoprotective strategies.
15520809 - Molecular mechanisms of caspase regulation during apoptosis.
11232179 - Cell death at the millennium. implications for liver diseases.
14634119 - Sustained nitric oxide delivery delays nitric oxide-dependent apoptosis in macrophages:...
25446569 - Naringenin reduces cholesterol-induced hepatic inflammation in rats by modulating matri...
23749739 - Immunosuppressive mechanisms of regulatory dendritic cells in cancer.
18625909 - Live lactobacillus rhamnosus and streptococcus pyogenes differentially regulate toll-li...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Molecular biotechnology     Volume:  20     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-08     Completed Date:  2002-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-14     Citation Subset:  IM    
Affiliation:
Dept. of Experimental Pathology and Oncology. Universita degli Studi di Firenze, Italy. l.magnelli@unifi.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / metabolism
Apoptosis*
Apoptotic Protease-Activating Factor 1
Cell Death
DNA Damage
Flow Cytometry
Hematologic Neoplasms / pathology*
Humans
In Situ Nick-End Labeling
Proteins / metabolism*
Chemical
Reg. No./Substance:
0/APAF1 protein, human; 0/Antibodies, Monoclonal; 0/Apoptotic Protease-Activating Factor 1; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Improving fluorescence-based assays for the in vitro analysis of cell adhesion and migration.
Next Document:  Universal primers for the PCR-mediated amplification of DNA beta: a molecule associated with some mo...