| Apoptosis modulation of Epstein-Barr virus-encoded latent membrane protein 1 in the epithelial cell line HeLa is stimulus-dependent. | |
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MedLine Citation:
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PMID: 12504573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is required for viral transformation and has been shown to protect lymphocytes from apoptosis. However, the effect of LMP1 on cells of epithelial origin remains poorly understood. Using the epithelial cell line HeLa in which the expression of LMP1 is inducibly regulated by tetracycline, we demonstrate that apoptosis triggered by ligation of the death receptor, Fas, or by the chemotherapeutic agent, etoposide, is potentiated by LMP1. Apoptosis was assessed by nuclear condensation and activation of caspase-3-like enzymes with concomitant proteolysis of the nuclear caspase substrate, poly(ADP-ribose) polymerase. However, the effect of LMP1 in HeLa cells appeared to be stimulus-dependent since apoptosis induced by tumor necrosis factor (TNF) was inhibited. Moreover, we observed an upregulation of the zinc finger protein A20 and a decrease in expression of Bcl-2 upon induction of LMP1 in HeLa cells. Taken together, these data further our understanding of the function of LMP1 in epithelial cells and suggest that LMP1, similar to its mammalian homolog CD40, can exert opposing effects on cell survival depending on the nature of the apoptosis trigger. |
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Authors:
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Xiangning Zhang; LiFu Hu; Bengt Fadeel; Ingemar T Ernberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virology Volume: 304 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-30 Completed Date: 2003-05-08 Revised Date: 2008-01-09 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 330-41 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Elsevier Science (USA) |
Affiliation:
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Microbiology and Tumor Biology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD95
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physiology Apoptosis* Caspase 3 Caspases / physiology Etoposide / pharmacology Hela Cells Humans Intracellular Signaling Peptides and Proteins Nasopharyngeal Neoplasms / virology Nuclear Proteins Protein Biosynthesis Proteins* Proto-Oncogene Proteins c-bcl-2 / biosynthesis Tumor Necrosis Factor-alpha / pharmacology Viral Matrix Proteins / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/EBV-associated membrane antigen, Epstein-Barr virus; 0/Intracellular Signaling Peptides and Proteins; 0/Nuclear Proteins; 0/Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Necrosis Factor-alpha; 0/Viral Matrix Proteins; 33419-42-0/Etoposide; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases; EC 6.3.2.19/TNFAIP3 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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