| Apoptosis induction by inhibitors of Ser/Thr phosphatases 1 and 2A is associated with transglutaminase activation in two different human epithelial tumour lines. | |
| | |
MedLine Citation:
|
PMID: 9287133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two epithelial tumour lines, HeLa and KB, were treated with okadaic acid and calyculin A, specific inhibitors of Ser/Thr phosphatases (PP), esp. PP1 and PP2A. Morphological criteria, analysis of DNA fragmentation and studies of membrane integrity revealed that both agents concentration- and time-dependently induced apoptosis at nanomolar concentrations which in these cells was associated with the stimulation of a transglutaminase activity. Since a non-functional derivative of okadaic acid did not affect cell viability apoptosis was apparently related to the inhibition of PP1 and PP2A. Membrane damage marker activity was delayed by at least 24 h when compared to nuclear alterations. |
| | |
Authors:
|
C von Zezschwitz; H Vorwerk; F Tergau; H J Steinfelder |
Related Documents
:
|
10753663 - Two acidic amino acid residues, asp(470) and glu(471), contained in the carboxyl cytopl... 6859103 - Changes of serum hexosaminidase for the presumptive diagnosis of type i gaucher disease... 10888583 - Simple method for large scale isolation of the cyclic arylhydroxamic acid dimboa from m... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: FEBS letters Volume: 413 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Aug |
Date Detail:
|
Created Date: 1997-10-30 Completed Date: 1997-10-30 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 147-51 Citation Subset: IM |
Affiliation:
|
Institute of Pharmacology and Toxicology, University of Göttingen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis* Cell Membrane / drug effects, enzymology DNA Fragmentation Dose-Response Relationship, Drug Enzyme Activation Epithelium / drug effects, enzymology, physiology Hela Cells Humans L-Lactate Dehydrogenase / metabolism Okadaic Acid / pharmacology Oxazoles / pharmacology Phosphoprotein Phosphatases / antagonists & inhibitors, physiology* Time Factors Transglutaminases / metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Oxazoles; 101932-71-2/calyculin A; 78111-17-8/Okadaic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.3.2.13/Transglutaminases; EC 3.1.3.16/Phosphoprotein Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Amino acid substitutions in the first transmembrane domain (TM1) of P-glycoprotein that alter substr...
Next Document: The protein kinase C pseudosubstrate peptide (PKC19-36) inhibits insulin-stimulated protein kinase a...