Document Detail


Apoptosis-inducing effect of the DR5 monoclonal antibody, D-6, alone or in combination with cisplatin, on A2780 ovarian cancer cells.
MedLine Citation:
PMID:  22562097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Death receptor 5 (DR5) antibody (D-6) is a monoclonal antibody directed against DR5. The aim of this study was to explore the apoptosis-inducing effects of DR5 (D-6), alone or in combination with cisplatin, on A2780 ovarian cancer cells. The cells were treated with various concentrations of cisplatin and/or DR5 (D-6), or a combination of both. For the control group, the cells were treated only with culture medium. Twenty-four hours after the culture, the morphological changes of each group were observed under an inverted microscope. The cell growth inhibition rates were also analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assays, and the apoptosis was analyzed by flow cytometry. Western blotting analysis was used to detect the intracellular reaction. Our results showed that DR5 monoclonal antibody (D-6) was able to induce the apoptosis and increase the cell growth inhibition rates of ovarian cancer cells in a dose-dependent manner, and the effect was enhanced by cisplatin. There were significant morphological changes, a higher cell growth inhibition rate and apoptosis rate and lesser expression of caspase-3, 8, 9 precursors in the cells treated with both cisplatin and DR5 (D-6). The combination treatment of the DR5 monoclonal antibody (D-6) with cisplatin may be a promising treatment for ovarian cancer.
Authors:
Qin Jiang; Hong Zhu; Baoquan Liang; Yan Huang; Chunmei Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-03
Journal Detail:
Title:  Molecular medicine reports     Volume:  6     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-06-19     Completed Date:  2012-10-16     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  316-20     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal, Murine-Derived / pharmacology*
Antineoplastic Combined Chemotherapy Protocols / pharmacology*
Apoptosis*
Blotting, Western
Caspase 8 / metabolism
Cell Line, Tumor
Cisplatin / pharmacology*
Culture Media / metabolism
Drug Screening Assays, Antitumor
Female
Flow Cytometry
Mice
Ovarian Neoplasms / metabolism*,  pathology
Receptors, TNF-Related Apoptosis-Inducing Ligand / immunology*
Signal Transduction
TNF-Related Apoptosis-Inducing Ligand / metabolism
Tetrazolium Salts / metabolism
Thiazoles / metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal, Murine-Derived; 0/Culture Media; 0/Receptors, TNF-Related Apoptosis-Inducing Ligand; 0/TNF-Related Apoptosis-Inducing Ligand; 0/Tetrazolium Salts; 0/Thiazoles; 15663-27-1/Cisplatin; 298-93-1/thiazolyl blue; EC 3.4.22.-/Casp8 protein, mouse; EC 3.4.22.-/Caspase 8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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