| Apoptosis-inducing effect of the DR5 monoclonal antibody, D-6, alone or in combination with cisplatin, on A2780 ovarian cancer cells. | |
| | |
MedLine Citation:
|
PMID: 22562097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Death receptor 5 (DR5) antibody (D-6) is a monoclonal antibody directed against DR5. The aim of this study was to explore the apoptosis-inducing effects of DR5 (D-6), alone or in combination with cisplatin, on A2780 ovarian cancer cells. The cells were treated with various concentrations of cisplatin and/or DR5 (D-6), or a combination of both. For the control group, the cells were treated only with culture medium. Twenty-four hours after the culture, the morphological changes of each group were observed under an inverted microscope. The cell growth inhibition rates were also analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assays, and the apoptosis was analyzed by flow cytometry. Western blotting analysis was used to detect the intracellular reaction. Our results showed that DR5 monoclonal antibody (D-6) was able to induce the apoptosis and increase the cell growth inhibition rates of ovarian cancer cells in a dose-dependent manner, and the effect was enhanced by cisplatin. There were significant morphological changes, a higher cell growth inhibition rate and apoptosis rate and lesser expression of caspase-3, 8, 9 precursors in the cells treated with both cisplatin and DR5 (D-6). The combination treatment of the DR5 monoclonal antibody (D-6) with cisplatin may be a promising treatment for ovarian cancer. |
| | |
Authors:
|
Qin Jiang; Hong Zhu; Baoquan Liang; Yan Huang; Chunmei Li |
Related Documents
:
|
15376947 - Laser ablation patterning by interference induces directional cell growth. 21175587 - Mitochondrial genome depletion dysregulates bile acid- and paracetamol-induced expressi... 826887 - On the role of tumor cell surface in the accumulation of radioisotopes. 22278727 - Effects of symptomatic and asymptomatic isolates of blastocystis hominis on colorectal ... 12803587 - Respiratory burst activity of polymorphonuclear cells is dependent on the cell preparat... 6858687 - Gastrin production following antrectomy. a study on the occurrence of gastrin-producing... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-03 |
Journal Detail:
|
Title: Molecular medicine reports Volume: 6 ISSN: 1791-3004 ISO Abbreviation: Mol Med Rep Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-06-19 Completed Date: 2012-10-16 Revised Date: 2013-02-22 |
Medline Journal Info:
|
Nlm Unique ID: 101475259 Medline TA: Mol Med Rep Country: Greece |
Other Details:
|
Languages: eng Pagination: 316-20 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal, Murine-Derived / pharmacology* Antineoplastic Combined Chemotherapy Protocols / pharmacology* Apoptosis* Blotting, Western Caspase 8 / metabolism Cell Line, Tumor Cisplatin / pharmacology* Culture Media / metabolism Drug Screening Assays, Antitumor Female Flow Cytometry Mice Ovarian Neoplasms / metabolism*, pathology Receptors, TNF-Related Apoptosis-Inducing Ligand / immunology* Signal Transduction TNF-Related Apoptosis-Inducing Ligand / metabolism Tetrazolium Salts / metabolism Thiazoles / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal, Murine-Derived; 0/Culture Media; 0/Receptors, TNF-Related Apoptosis-Inducing Ligand; 0/TNF-Related Apoptosis-Inducing Ligand; 0/Tetrazolium Salts; 0/Thiazoles; 15663-27-1/Cisplatin; 298-93-1/thiazolyl blue; EC 3.4.22.-/Casp8 protein, mouse; EC 3.4.22.-/Caspase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Analysis of macroautophagy by immunohistochemistry.
Next Document: Case-control study of orf in preweaned lambs and an assessment of the financial impact of the diseas...