| Apoptosis-inducing active components from Corbicula fluminea through activation of caspase-2 and production of reactive oxygen species in human leukemia HL-60 cells. | |
| | |
MedLine Citation:
|
PMID: 16545898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The anti-cancer effects and possible mechanisms of the freshwater clam (Corbicula fluminea Muller) and its active compounds (FME) on cell viability in human leukemia HL-60 cells were investigated. This study demonstrated that FME was able to inhibit cell proliferation in a concentration- and time-dependent manner. Treatment with FME caused induction of caspase-2, caspase-3, caspase-6, caspase-8, and caspase-9 activity in a time-dependent manner, but not affect caspase-1 activity; it induced the proteolysis of DNA fragmentation factor (DFF-45) and poly(ADP-ribose) polymerase (PARP). Induction of cell death by FME was completely prevented by a pan-caspase inhibitor, Z-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK) and a caspase-2 inhibitor, Z-Val-Asp-Val-Ala-Asp-FMK (Z-VDVAD-FMK). Furthermore, treatment with FME caused a rapid loss of mitochondrial transmembrane potential, stimulation of generation of reactive oxygen species (ROS), release of mitochondrial cytochrome c into cytosol, and GSH depletion. Anti-oxidants such as N-acetylcysteine, catalase, superoxide dismutase, allopurinol, and pyrrolidine dithiocarbamate, but not diphenylene iodonium, significantly inhibited FME-induced cell death. In addition, the results showed that FME-induced apoptosis was accompanied by up-regulation of Bax and Bad, and down-regulation of Bcl-2 and Bcl-XL. Taken together, induction of apoptosis on HL-60 cells by FME was mainly associated with ROS production, GSH depletion, mitochondrial dysfunction, and caspase activation. |
| | |
Authors:
|
Ying-Tang Huang; Yi-Hsuan Huang; Tzhy-Chyuan Hour; Bonnie Sun Pan; Yeuk-Chuen Liu; Min-Hsiung Pan |
Related Documents
:
|
17487878 - Caspase-3 activation in astrocytes following postnatal excitotoxic damage correlates wi... 20138848 - Distribution of apoptosis-related proteins in sporadic creutzfeldt-jakob disease. 17013758 - Caspase-3 mediated feedback activation of apical caspases in doxorubicin and tnf-alpha ... 9164928 - Dissociation of apoptosis and activation of il-1beta-converting enzyme/ced-3 proteases ... 19127268 - Lll-3 inhibits stat3 activity, suppresses glioblastoma cell growth and prolongs surviva... 22371398 - Murine membranous nephropathy: immunization with α3(iv) collagen fragment induces sube... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-20 |
Journal Detail:
|
Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 44 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2006 Aug |
Date Detail:
|
Created Date: 2006-06-23 Completed Date: 2006-09-19 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
|
Languages: eng Pagination: 1261-72 Citation Subset: IM |
Affiliation:
|
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Chloromethyl Ketones
/
pharmacology Animals Antioxidants / pharmacology Apoptosis / drug effects* Caspase 2 Caspases / antagonists & inhibitors, metabolism* Cell Growth Processes / drug effects Cell Survival / drug effects Corbicula / chemistry* Cytochromes c / secretion Drug Interactions Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Flow Cytometry Glutathione / metabolism HL-60 Cells Humans Isoenzymes Membrane Potentials / drug effects Mitochondrial Membranes / drug effects, physiology Proto-Oncogene Proteins c-bcl-2 / biosynthesis Reactive Oxygen Species / antagonists & inhibitors, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acid Chloromethyl Ketones; 0/Antioxidants; 0/Enzyme Inhibitors; 0/Isoenzymes; 0/Proto-Oncogene Proteins c-bcl-2; 0/Reactive Oxygen Species; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; 70-18-8/Glutathione; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 2; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Adult attachment patterns and individual psychotherapy: a review.
Next Document: Inducibility of UDP-glucuronosyltransferase 1As by beta-naphthoflavone in HepG2 cells.