Document Detail

Apoptosis induced by oxalate in human renal tubular epithelial HK-2 cells.
MedLine Citation:
PMID:  15759146     Owner:  NLM     Status:  MEDLINE    
Oxalate is not only considered to be one of the main constituents of urinary stones, but it also has toxic effects on renal tubular epithelial cells, affecting the pathogenesis of nephrolithiasis. We tried to elucidate the effects of oxalate on human renal tubular epithelial cells (HK-2 cells). In addition, we investigated whether the toxic effect of oxalate occurs by apoptosis, and determined the expression of Bcl-2 family and caspase 9 proteins known as apoptosis-related protein. HK-2 cells were incubated with different concentrations of oxalate, and the effect of oxalate on the growth of the cells was assessed by MTT assay. A caspase-3 activity assay and TUNEL assay were performed on HK-2 cells after oxalate exposure in order to evaluate apoptosis. Immunoblot analysis of Bax, Bcl-2, Bcl-xL, and caspase-9 were performed. Oxalate exposure resulted in significant growth inhibition of HK-2 cells as oxalate concentrations increased. The toxic effect of oxalate on HK-2 cells was considered to occur through apoptosis, as suggested by the increase of caspase-3 activity. The percentage of positive nuclei stained using the TUNEL method was 18+/-2.3 in oxalate-treated cells and 2.5+/-0.9 in untreated cells (P<0.05). Bax and caspase-9 protein expression increased significantly as oxalate concentrations increased, but Bcl-2 protein expression decreased. There was no difference in Bcl-xL protein expression among the various concentrations of oxalate. Our results show that oxalate has a toxic effect on HK-2 cells and that this effect is induced by apoptosis, which may be mediated by an intrinsic pathway.
Byong-Chang Jeong; Cheol Kwak; Kyu Seon Cho; Bong Sub Kim; Sung Kyu Hong; Jung-In Kim; Chongwook Lee; Hyeon Hoe Kim
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Publication Detail:
Type:  Journal Article     Date:  2005-03-10
Journal Detail:
Title:  Urological research     Volume:  33     ISSN:  0300-5623     ISO Abbreviation:  Urol. Res.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-06-13     Completed Date:  2005-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364311     Medline TA:  Urol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  IM    
Department of Urology, Seoul National University College of Medicine and Clinical Research Institute, Seoul National University Hospital, 28, Yongon-dong, Chongno-gu, Seoul 110-744, Korea.
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MeSH Terms
Caspase 9
Caspases / analysis,  metabolism
Cell Proliferation / drug effects
Epithelial Cells / chemistry,  drug effects,  metabolism
Kidney Tubules / cytology*
Oxalates / toxicity*
Proto-Oncogene Proteins c-bcl-2 / analysis,  metabolism
Urothelium / chemistry,  drug effects,  metabolism
Reg. No./Substance:
0/Oxalates; 0/Proto-Oncogene Proteins c-bcl-2; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases

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