Document Detail


Apoptosis induced by emodin is associated with alterations of intracellular acidification and reactive oxygen species in EC-109 cells.
MedLine Citation:
PMID:  20651850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Emodin (1,3,8-trihydroxy-6-methylanthraquinone), a natural anthraquinone derivative found in several herbal medicines, is highly active in suppressing the proliferation of various tumor cells such as breast, hepatocellular, and lung cancer cells under in vitro conditions. The mechanism of emodin-induced apoptosis in esophagus carcinoma cells, EC-109, is not completely understood. In this study, EC-109 cells treated with emodin underwent rapid apoptosis as judged by morphological changes and flow cytometry analysis. The addition of emodin to EC-109 cells led to the inhibition of growth in a time- and dose-dependent manner. Fluorescence measurements of cells indicated that the intracellular pH (pHi) decreased significantly by 0.47-0.78 units. The results obtained from flow cytometry suggested that bursts of reactive oxygen species took place after the application of emodin. The present study indicates that emodin may be a strong anticancer drug against esophagus cancer cells by causing various early events leading to growth inhibition, including the production of reactive oxygen species and decrease of pHi, which may result in cellular apoptosis.
Authors:
Q J Wang; X B Cai; M H Liu; H Hu; X J Tan; X B Jing
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  88     ISSN:  1208-6002     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  767-74     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, The First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism*
Antineoplastic Agents, Phytogenic / pharmacology
Apoptosis / drug effects*
Carcinoma / metabolism,  pathology*
Cell Line, Tumor
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Emodin / pharmacology*
Esophageal Neoplasms / metabolism,  pathology*
Flow Cytometry
Humans
Hydrogen-Ion Concentration
Intracellular Fluid / drug effects,  metabolism
Reactive Oxygen Species / metabolism*
Up-Regulation / drug effects
Chemical
Reg. No./Substance:
0/Acids; 0/Antineoplastic Agents, Phytogenic; 0/Reactive Oxygen Species; 518-82-1/Emodin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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