Document Detail


Apoptosis induced by (di-isopropyloxyphoryl-Trp)2-Lys-OCH3 in K562 and HeLa cells.
MedLine Citation:
PMID:  18376070     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
According to the method used in our laboratory,our group synthesized (DIPP-Trp)2-Lys-OCH 3. It inhibited the proliferation of K562 and HeLa cells in a dose-and time-dependent manner with an IC 50 of 15.12 and 42.23 microM, respectively. (DIPP-Trp) 2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells;the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter,USA). Phosphatidylserine could signi?cantly translocate to the surface of the membrane in (DIPP-Trp)2-Lys-OCH3-treated K562 and HeLa cells. The increase of an early apoptotic population was observed in a dose-dependent manner by both annexin-FITC and PI staining. It was concluded that (DIPP-Trp) 2-Lys-OCH3 not only induced cells to enter into apoptosis,but also affected the progress of the cell cycle. It may have arrested the K562 and HeLa cells in the G 2/M,S phases,respectively. The apoptotic pathway was pulsed at this point,resulting in the treated cells entering into programmed cell death.(DIPP- Trp)-Lys-OCH is a potential anticancer drug that intervenes in the signalling pathway.
Authors:
Feng Liu; Shi-Ying Liu; Ping Xu; Zheng-Hua Xie; Guo-Ping Cai; Yu-Yang Jiang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biosciences     Volume:  33     ISSN:  0250-5991     ISO Abbreviation:  J. Biosci.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-08-21     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  55-62     Citation Subset:  IM    
Affiliation:
The Key Laboratory of Chemical Biology, Guangdong Province, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
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MeSH Terms
Descriptor/Qualifier:
Annexins / metabolism
Apoptosis / drug effects*,  physiology
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Flow Cytometry
Fluorescein-5-isothiocyanate / metabolism
Fluorescent Dyes / metabolism
G2 Phase / drug effects
Hela Cells
Humans
Inhibitory Concentration 50
K562 Cells
Mitosis / drug effects
Molecular Structure
Oligopeptides / chemical synthesis,  chemistry,  pharmacology*
Phosphopeptides / chemical synthesis,  chemistry,  pharmacology*
S Phase / drug effects
Tetrazolium Salts / analysis,  metabolism
Thiazoles / analysis,  metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Annexins; 0/Fluorescent Dyes; 0/Oligopeptides; 0/Phosphopeptides; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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