| Apoptosis in thalassemia major reduced by a butyrate derivative. | |
| | |
MedLine Citation:
|
PMID: 16227679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND AND OBJECTIVES: In cases of beta-thalassemia major, apoptosis appears to be greatly enhanced in the early-stage erythroid precursors in the bone marrow leading to ineffective erythropoiesis. L-Carnitine is found to strongly reduce apoptosis in different diseases. We investigated the effect of oral L-carnitine therapy on apoptosis in thalassemia major patients. METHODS: Eighteen thalassemia major patients with a mean age of 12.2 +/- 6.6 years were included. Detection of apoptosis was done by photometric enzyme immunoassay (ELISA) and agarose gel electrophoresis before and after 6 months of oral therapy with L-carnitine (50 mg/kg/day). RESULTS: A significant decrease of apoptosis frequency in the erythroid precursors in the bone marrow of studied cases was noted after therapy. The quantity of nucleosomes measured by ELISA dropped from 3.65 +/- 1.338 to 1.60 +/- 0.65 after therapy (p = 0.005). A positive ladder pattern reflecting apoptosis on agarose gel electrophoresis was detected in 88.9% of cases prior to treatment versus 16.7% after therapy (p = 0.006). Patients also had a significant decrease in the frequency of transfusions and increase in the pre-transfusion hemoglobin levels after therapy. CONCLUSION: L-Carnitine seems to be a good modulator of apoptotic processes in thalassemic patients leading to a decreased frequency of programmed erythroblast death and general improvement of the disease condition. |
| | |
Authors:
|
A El-Beshlawy; H Seoud; A Ibrahim; I Youssry; H Gabre; H Isma'eel; E Aoun; A Taher |
Related Documents
:
|
20591689 - Increased th17 rather than th1 alloimmune response is associated with cardiac allograft... 17245639 - Rapid cold-hardening protects drosophila melanogaster from cold-induced apoptosis. 20936709 - In vivo optical imaging of early-stage apoptosis in mouse brain after transient cerebra... 19128239 - Peroxiredoxin 5 confers protection against oxidative stress and apoptosis and also prom... 12725289 - Induction of apoptosis in macrophage cell line, j774, by the cell-free supernatant from... 9648109 - Measurement of cell death. 23090959 - Genome expression analysis of non-proliferating intracellular salmonella unravels an ac... 3555299 - Lung inflammation: normal host defense or a complication of some diseases? 23379349 - Infiltration of myeloid cells into decidua is a critical early event in the labour casc... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Acta haematologica Volume: 114 ISSN: 0001-5792 ISO Abbreviation: Acta Haematol. Publication Date: 2005 |
Date Detail:
|
Created Date: 2005-10-17 Completed Date: 2005-11-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0141053 Medline TA: Acta Haematol Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 155-9 Citation Subset: IM |
Copyright Information:
|
(c) 2005 S. Karger AG, Basel |
Affiliation:
|
Pediatric Department, School of Medicine and Al Azhar Universities, Cairo, Egypt. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Apoptosis / drug effects* Bone Marrow Cells / drug effects, pathology Carnitine / therapeutic use* Child Child, Preschool DNA Fragmentation / drug effects Erythroblasts / drug effects, pathology Female Hematologic Agents / therapeutic use* Humans Male Nucleosomes / drug effects, pathology beta-Thalassemia / drug therapy*, pathology* |
| Chemical | |
Reg. No./Substance:
|
0/Hematologic Agents; 0/Nucleosomes; 541-15-1/Carnitine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tyrosine kinase inhibitor STI571 (Imatinib) cooperates with wild-type p53 on K562 cell line to enhan...
Next Document: CD56-positive acute lymphoblastic leukemia.