| Apoptosis in the monkey small intestinal epithelium: structural and functional alterations in the mitochondria. | |
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MedLine Citation:
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PMID: 10232826 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our earlier studies have shown apoptosis in the villus tip cells of the monkey small intestinal epithelium. Because mitochondria have been implicated in the apoptotic process, this study looked at the function and lipid composition of mitochondria isolated from apoptotic villus tip cells and compared it with middle and crypt cells. Decreased MTT reduction and respiratory control ratio, increased swelling and altered mitochondrial enzyme activities were seen in the villus tip cell mitochondria when compared to other cells. The lipid composition of the villus tip mitochondria were different from the other mitochondria. A decrease in phosphatidylethanolamine and phosphatidyl-inositol and an increase in phosphatidic acid was seen in these mitochondria. Fatty acid composition analysis showed more unsaturated fatty acids in the free fatty acid and phospholipid fraction in villus tip cell mitochondria as compared to other cells. These studies suggest that in the monkey small intestinal epithelium, apoptotic process is associated with functional and structural alterations in the mitochondria. |
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Authors:
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M Madesh; R Anup; O Benard; K A Balasubramanian |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical biology & medicine Volume: 26 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-06-23 Completed Date: 1999-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 836-43 Citation Subset: IM |
Affiliation:
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Department of Gastrointestinal Sciences, Christian Medical College and Hospital, Vellore, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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metabolism Animals Apoptosis* Cholesterol / metabolism Fatty Acids, Nonesterified / metabolism Glycerides / metabolism Haplorhini Intestinal Mucosa / cytology*, physiology* Intestine, Small Isocitrate Dehydrogenase / metabolism Lipid Metabolism* Mitochondria / metabolism* Mitochondrial Swelling NADH Dehydrogenase / metabolism Oxygen Consumption* Phospholipids / metabolism* Succinate Dehydrogenase / metabolism Succinates / metabolism Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Glycerides; 0/Phospholipids; 0/Succinates; 57-88-5/Cholesterol; EC 1.1.1.41/Isocitrate Dehydrogenase; EC 1.15.1.1/Superoxide Dismutase; EC 1.3.99.1/Succinate Dehydrogenase; EC 1.6.99.3/NADH Dehydrogenase; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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