Document Detail


Apoptosis in gallbladder carcinomas and dysplasias, its relation to the expression of caspases 3, 6 and 8 and apoptosis regulating proteins bcl-2, mcl-1 and bax.
MedLine Citation:
PMID:  10668195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we investigated apoptosis and the expression of caspases 3, 6 and 8 and bcl-2, mcl-1 and bax in 39 gallbladder carcinomas and 7 epithelial dysplasias. The average apoptotic index was 0.68 +/- 0.91%. The extent of apoptosis was higher in grade II-III than grade I tumours or epithelial dysplasias (p = 0.003). Also, tumours invading beyond serosa or into other organs (T3-T4) had a higher apoptotic index than other tumours (p = 0.05). Caspase 3 expression was found in 37 (95%) and caspase 6 and 8 expression each in 30 (77%) carcinomas. Their expression associated with each other and tended to increase along with the progression of the lesions. Bcl-2 expression was found in only 4 (10%) tumours. In contrast, mcl-1 positivity was found in 34 (87%) and bax positivity in all cases. The results show that apoptosis is increased along with progression of the neoplastic lesions of the gallbladder epithelium. Caspases 3, 6 and 8 are strongly expressed in gallbladder carcinomas suggesting that they contribute to the increased apoptosis observed in them. Of the bcl-2 family proteins, bcl-2 was expressed infrequently suggesting that it does not play any significant role in apoptosis inhibition in gallbladder tumours.
Authors:
N Turunen; P Pääkkö; Y Soini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  15     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-29     Completed Date:  2000-02-29     Revised Date:  2008-07-09    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  53-60     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Oulu, Finland.
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MeSH Terms
Descriptor/Qualifier:
Caspase 3
Caspase 6
Caspase 8
Caspase 9
Caspases / analysis*
Gallbladder / enzymology,  pathology*
Gallbladder Neoplasms / enzymology*,  pathology*
Humans
Necrosis
Neoplasm Invasiveness
Neoplasm Proteins / analysis*
Proto-Oncogene Proteins / analysis*
Proto-Oncogene Proteins c-bcl-2 / analysis*
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/BAX protein, human; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 0/myeloid cell leukemia sequence 1 protein; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP6 protein, human; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 6; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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