Document Detail

Apoptosis in fetal nucleated erythrocytes circulating in maternal blood.
MedLine Citation:
PMID:  11113889     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine if apoptosis occurs in fetal cells that have crossed into the maternal circulation, which would potentially explain the difference between the number of intact fetal cells and the amount of fetal DNA detectable in maternal plasma. We flow-sorted fetal nucleated erythrocytes (FNRBCs) using antibody to the gamma chain of fetal haemoglobin and confirmed them to be fetal in origin by FISH analysis using chromosome-specific probes. Fetal cells were then analysed microscopically for the presence of terminal UdTP nuclear end labelling (TUNEL) staining. Apoptotic change was observed in 42.7% of fetal NRBCs (106/246) and 3.5% of maternal cells (29/818). Results of this study indicate that a significant number of fetal cells in maternal blood are undergoing apoptosis at the time of sampling. Apoptosis may be one mechanism by which fetal cells are cleared by the maternal circulation.
A Sekizawa; O Samura; D K Zhen; V Falco; A Farina; D W Bianchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  20     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-18     Completed Date:  2001-01-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  886-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Division of Genetics, Department of Pediatrics, New England Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA.
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MeSH Terms
Cell Separation
Erythroblasts / pathology*
Fetal Blood / cytology*
Flow Cytometry
In Situ Hybridization, Fluorescence
In Situ Nick-End Labeling
Maternal-Fetal Exchange*
Pregnancy Trimester, First
Pregnancy Trimester, Second
Single-Blind Method
Y Chromosome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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