| Apoptosis in the absence of poly-(ADP-ribose) polymerase. | |
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MedLine Citation:
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PMID: 9144569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cleavage of poly-(ADP-ribose) polymerase is a process occurring early during the execution phase of apoptosis. Although in many experimental systems PARP cleavage indicates a point of no return, the significance of this proteolytic step for apoptosis remains unclear. Here we compare the susceptibility of cells from wild-type mice and PARP-/- mice to several inducers of apoptosis. Neither the susceptibility of hepatocytes towards CD95 or TNF-mediated apoptosis nor the activation of PARP-cleaving caspases was modified in PARP-/- liver cells. Thymocytes with either genotype exhibited similar sensitivity to treatments with ceramide, dexamethasone, or etoposide. The sensitivity of primary neurons towards apoptosis induced by staurosporine, colchicine, potassium withdrawal, peroxynitrite, or the neurotoxin MPP+ was also unaltered. These data suggest that neither activation nor cleavage of PARP has a causal role in apoptotic cell death of primary, non-transformed cells. |
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Authors:
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M Leist; B Single; G Künstle; C Volbracht; H Hentze; P Nicotera |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 233 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-06-05 Completed Date: 1997-06-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 518-22 Citation Subset: IM |
Affiliation:
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Department of Molecular Toxicology, University of Konstanz, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Cells, Cultured Electrophoresis, Agar Gel L-Lactate Dehydrogenase / metabolism Liver / metabolism Mice Microscopy, Fluorescence Poly(ADP-ribose) Polymerases / metabolism* Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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